动物医学进展2023，Vol.44Issue(12)：17-23,7.
1株黑天鹅源H5N8亚型禽流感病毒遗传演化分析
Genetic Evolution Analysis of A H5N8 Subtype Avian Influenza Virus Isolated from Black Swan
摘要
Abstract
To analyze the genetic evolution characteristics of a strain of H5N8 subtype avian influenza virus(AIV)iso-lated from black swan,the complete genomic sequences of a strain of H5N8 subtype avian influenza virus(A/Wild swan/China/Beijing0201/2021)identified from the black swan samples collected by Beijing Yuanmingyuan park in 2021 were amplified,cloned and sequenced.The genetic evolution and key amino acid sites were analyzed by molecular biology software.The results showed that the H5N8 subtype avian influenza virus strain belonged to clade 2.3.4.4b evo-lutionary branch.The PB2,PB1,PA,HA,NP,NA,MP and NS genes were highly homologous with the corresponding genes of H5N8 subtype AIV,and the strain was closely related to the H5N8 strains found in Vietnam,Kazakhstan,Russia,Cambodia and France from 2020 to 2021.Amino acid site analysis showed that the HA protein cleavage site of this strain had the molecular characteristics of highly pathogenic avian influenza virus,contained the amino mutations of S137A,T160A,T192I and S227R,with the deletion of glycosylation sites at the 158th position,and mutations of these sites could enhance the binding ability of H5 subtype avian influenza virus to human sialic acid receptors.The PB2 protein,NP protein,M1 protein and NS1 protein of this strain all had several key amino acid sites that can enhance the replication ability and pathogenicity of influenza virus in mammals.This study provided experimental basis for the stud-y on the evolution and comprehensive prevention and control of H5N8 subtype avian influenza virus.关键词
黑天鹅/禽流感病毒/H5N8亚型/遗传演化Key words
black swan/avian influenza virus/H5N8 subtype/genetic evolution分类
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栗云鹏,刘海莹,张启龙,刘晓冬,周德刚,张跃,傅彩霞..1株黑天鹅源H5N8亚型禽流感病毒遗传演化分析[J].动物医学进展,2023,44(12):17-23,7.基金项目
北京市动物疫病监测及预警预报项目 （）
