动物医学进展2023，Vol.44Issue(12)：23-28,6.
不同注射剂量9型腺相关病毒载体在小鼠脑和脊髓表达效果分析
Analysis of Expression Effects of Different Injection Volumes of Adeno-associated Virus Type 9 Vector in Brain and Spinal Cord of Mice
摘要
Abstract
Adeno-associated virus(AAV),particularly AAV9 that migrates from terminal axons to neu-rons,is a powerful tool for studying neural circuits and brain function.Using a combination of vesicular glu-tamate transporter 2(VGlut2)gene knockout in mice and stereotactic brain injections,the AAV9-DIO-ChR2-EGFP virus was injected into the locus coeruleus.The study investigated the effects of different injec-tion doses on the range of viral infection and mouse weight.The results indicated that,compared to the higher injection volume(1 μL),the lower injection volume(0.5 μL)can confine AAV9-DIO-ChR2-EGFP to the locus coeruleus.Three weeks after injecting AAV9-DIO-ChR2-EGFP,the infection range extended from the locus coeruleus to the primary motor cortex(Ml),thalamus,araphe palidus nucleus(Rpa),and cervical spinal cord(CSC).Subsequent testing showed that the low dose of AAV9(0.5 μL)had no effect on mouse body weight(P=0.979 5).These results demonstrate that a low injection dose(0.5 μL)can par-tially limit the expression range of AAV and that intracranial AAV9 injection does not alter mouse body weight.关键词
腺相关病毒载体/立体定位注射/体重/中枢神经系统Key words
Adeno-associated virus vector/stereotactic injection/weight/central nervous system分类
农业科技引用本文复制引用
李鹏辉,曹晓娟,张小宇,杜晨光,刘昊东,李嘉成,樊奇,王星,李彩琴,杨子程,陈玉洁,海日汗..不同注射剂量9型腺相关病毒载体在小鼠脑和脊髓表达效果分析[J].动物医学进展,2023,44(12):23-28,6.基金项目
国家自然科学基金项目(32060204) （32060204）
内蒙古科技攻关计划项目(2021GG0199) （2021GG0199）
内蒙古自然科学基金项目(2020MS03035) （2020MS03035）
