动物医学进展2023，Vol.44Issue(12)：36-41,6.
河南省猪伪狂犬病病毒感染情况调查及gE基因遗传变异分析
Investigation on Porcine Pseudorabies Virus Infection and Analysis on Genetic Variation of gE Gene in Henan
摘要
Abstract
In order to understand the infection of porcine pseudorabies virus(PRV)in different regions,sites and groups in Henan,9 540 serological samples and 1 649 tissue samples were collected from 253 sites with different scales in 18 cities in Henan from November 2020 to October 2021.Serum antibody was detec-ted by ELISA kit,and PRV nucleic acid in tissue samples were detected by fluorescence PCR.In addition,gE gene amplification,sequencing and genetic variation analysis were carried out on some positive samples.Serological test results showed that gE antibodies against PRV in pigs were detected in different areas of Henan,the average individual positive rate of gE antibodies against PRV was 26.03％,and the positive rate at farms/herds level was 54.15％.The average individual and farm/herd positive rates of commercial gener-ation farms were the highest in different farms/herds.Among the pig herds at different growth stages,nursery piglets had the highest individual positive rate.In addition,the highest individual positive rate was in winter.Results of pathogenic detection of tissue samples showed that a total of 54 virus nucleic acid posi-tive strains were detected,with an average individual positive rate of 3.27％.Among them,4 PRV strains were sequenced and analyzed for genetic evolution.The nucleotide homology between the gE gene sequences of the 4 PRV strains was 99.5％-99.9％,the homology with the classical foreign strain was 99.2％-99.5％,and the homology with the domestic epidemic variant strain was 99.6％-99.9％.They belonged to the geno-type Ⅱ group,all of which were variant strains.The results showed that PRV infection in pig farms in Henan was relatively common,and the epidemic strain was a variant strain.This study will provide an im-portant data reference for the screening,purification and comprehensive prevention and control of PRV im-mune strains in pig farms in Henan.关键词
猪/伪狂犬病/血清学调查/病原学调查/变异Key words
pig/Pseudorabies/serological investigations/etiological investigation/variation分类
农业科技引用本文复制引用
王华俊,王东方,闫若潜,刘光辉,房大学,郭洛生..河南省猪伪狂犬病病毒感染情况调查及gE基因遗传变异分析[J].动物医学进展,2023,44(12):36-41,6.基金项目
河南省重大科技专项项目(221100110600) （221100110600）
河南省重点研发与推广专项项目(222102110121,222102110289,222102110227) （222102110121,222102110289,222102110227）
河南省现代农业产业技术体系项目(HARS-22-12-T) （HARS-22-12-T）
