动物医学进展2023，Vol.44Issue(12)：42-46,5.
非洲鸵鸟源芽囊原虫基因型鉴定及分析
Genotype Identification and Analysis of Blastocystis from Struthio camelus
摘要
Abstract
Blastocystis is an opportunistic pathogenic protozoan with worldwide distribution,to identify the infection status and the genotypes of Blastocystis that infected Struthio camelus in the Struthio camelus breeding profes-sional cooperatives in Yunnan province,90 faecal samples were collected to amplify the SSU rRNA gene of Blasto-cystis by nested PCR.The result showed that 62 samples were identified as the positive of Blastocystis;The detec-tion rate of Blastocystis was 68.89％(62/90),the highest infection rate was 86.36％(19/22)at 90 days of age group and the lowest infection rate was 6.25％(20/32)at 60 days of age group,there was no significant difference in the infection rate of Blastocystis among different age groups(P＞0.05,X2=6.67,df=3);Based on the sequence analysis of SSU rRNA gene,three subtypes of Blastocystis(ST5,ST7 and ST20)were identified.ST5 is the domi-nant subtype,and ST5,ST7 are the subtypes of zoonosis.This results enriched the genotyping data of Blastocystis from Struthio camelus in Yunnan province as well as provided reference for the prevention and control of Blasto-cystis.关键词
非洲鸵鸟/芽囊原虫/基因分型/核糖体小亚基Key words
Struthio camelus/Blastocystis/genotyping/small ribosomal subunit分类
农业科技引用本文复制引用
陈俊蓉,谢昕言,徐翠蓉,衡昭君,徐聪,王俊祥,杨建发,贺君君,吴志蕾..非洲鸵鸟源芽囊原虫基因型鉴定及分析[J].动物医学进展,2023,44(12):42-46,5.基金项目
云南省朱兴全专家工作站项目(202005AF150041) （202005AF150041）
云南农业大学兽医公共卫生省创新团队项目(202105AE160014) （202105AE160014）
