动物医学进展2023，Vol.44Issue(12)：60-65,6.
猫传染性腹膜炎病毒的检测及ORF 7ab、N和S基因遗传进化分析
Detection and Analysis of the Genetic Evolution of the ORF 7ab,N and S Genes of Feline Infectious Peritonitis Virus
摘要
Abstract
In this study,two cases of feline infectious peritonitis(FIP)were detected and diagnosed using autopsy,histopathology and immunohistochemistry(IHC),and the ORF 7ab,N and S genes of feline in-fectious peritonitis virus(FIPV)were amplified by RT-PCR in two tissue samples.The genes were se-quenced and subjected to genetic evolutionary analysis to elucidate the genetic evolutionary characteristics of the virus.The results showed that both cases presented with the typical gross lesions of mixed FIP,with an accumulation of plasma fibrinous exudate in the peritoneal cavity and scattered or diffuse distribution of small grey-white nodules on the peritoneal plasma surface.Histopathology showed the foci of inflammatory cell infiltration of variable size attached to the peritoneal surface,as well as necrotizing enterocolitis,inter-stitial pancreatitis and interstitial pneumonia.Strong positive signals specific for FPIV were seen in the foci of inflammatory cell infiltration,in the spleen and the pancreas.The genetic homology and genetic evolution analysis showed that the genes of the two cases belonged to FCoV type Ⅰ.IMAU No.11685 had the highest homology with ZJU1709 strain(MT239440)from Hangzhou,Zhejiang province,and was the closest rela-tive;IMAU No.11824 had the highest homology with QS strain(MW030108)from Wuhan,Hubei prov-ince,and was the closest relative.It indicateed that the two FIPV cases originated from a common ancestor with the domestic epidemic strain,in which the ORF 7ab and N genes are relatively conserved with insig-nificant variation,and some variation occurred in the S gene.It can provide data reference for the molecular epidemiological study and prevention and control of FIP in China.关键词
猫传染性腹膜炎/猫传染性腹膜炎病毒/免疫组织化学/遗传进化Key words
Feline infectious peritonitis/Feline infectious peritonitis virus/IHC/genetic evolution分类
农业科技引用本文复制引用
高雅,黄金田,冯心如,付胜,高娃,王文龙,丁玉林,王金玲..猫传染性腹膜炎病毒的检测及ORF 7ab、N和S基因遗传进化分析[J].动物医学进展,2023,44(12):60-65,6.基金项目
内蒙古自治区科技重大专项项目(2021ZD0012) （2021ZD0012）
