李美玲 1魏谭军 1杨芝芳 1陈飞 1黄爱苹 1唐启荣 1肖成 1罗进芳2
作者信息
- 1. 达州市中西医结合医院,四川达州 635000
- 2. 贵州中医药大学,贵阳 550025
- 折叠
摘要
Abstract
Objective:To establish the high-performance liquid chromatography combined with quantitative analysis of multi-components by single-marker(HPLC-QAMS)technology for the quantitative control of 8 kinds of components in Xiao'er Kesouning syrup,including mulberroside A,morusin M,dihydromorin,baicalin,wogonoside,praeruptorin A,praeruptorin B and praeruptorin E.Methods:Ultrasil C18 column was used with the mobile phase of acetonitrile(A)and 1 mL/L glacial acetic acid.The flow rate was 1.0 mL/min with a linear gradient elution,detection wavelengths were set at 280 nm(mulberroside A,morusin M,dihydromorin,baicalin and wogonoside),and 321 nm(praeruptorin A,praeruptorin B and praeruptorin E),the column temperature was maintained at 25 ℃.Baicalin was used as the reference substance,relative correction factors for the other 7 kinds of components were established to calculate the content of mulberroside A,morusin M,dihydromorin,wogonoside,praeruptorin A,praeruptorin B and praeruptorin E.Results:A good linear relationship with peak area was observed within the range from 0.79 to 19.75 μg/mL for mulberroside A,0.56 to 14.00 μg/mL for moracin M,0.38 to 9.50 μg/mL for dihydromorin,26.55 to 663.75 μg/mL for baicalin,4.66 to 116.50 μg/mL for wogonoside,5.87 to 146.75 μg/mL for praeruptorin A,1.71 to 42.75 μg/mL for praeruptorin B and 2.39 to 59.75 μg/mL for praeruptorin E,respectively.The average recovery rates for each component at low,medium and high concentrations ranged from 96.83％to 100.14％,with relative standard deviation(RSD)≤ 1.42％(n=9).The results obtained by using the HPLC-QAMS technology showed no significant difference from those obtained by using the external standard method(ESM).Conclusion:HPLC-QAMS technology provides a fast and feasible multi-marker simultaneous quality evaluation model for Xiao'er Kesouning syrup.关键词
高效液相色谱一测多评法/小儿咳嗽宁糖浆/相对校正因子/含量测定Key words
high-performance liquid chromatography combined with quantitative analysis of multi-components by single-marker/Xiao'er Kesouning syrup/relative correction factor/content determination分类
医药卫生
