广西植物2023，Vol.43Issue(11)：1999-2007,9.DOI:10.11931/guihaia.gxzw202209020
茅尾海红树根际土壤可培养细菌多样性及抑菌活性研究
Research of antibacterial activity and culturable bacteria isolate from mangrove rhizosphere soil in Maowei Sea
摘要
Abstract
In order to find culturable bacteria resources and antibacterial activity strain in the rhizosphere soil of mangrove plants from the Maowei Sea,the rhizosphere soil of five mangrove plants(Rhizophora stylosa,Talipariti tiliaceum,Sonneratia apetala,Aegiceras corniculatum,and Pluchea indica)was cultured by enrichment media and then isolated the culturable bacteria.The culturable bacteria were purified by six different isolation media.The diversity of culturable bacteria was analyzed by the 16S rRNA gene sequence information.Antimicrobial activities(Staphylococcus epidermidis,methicillin-resistant S.aureus,and Pseudomonas aeruginosa)of the culturable bacteria were analyzed by disk diffusion method.The results were as follows:(1)A total of 120 strains were isolated from seven rhizosphere soil samples of mangrove plants which could be classified into 35 families and 47 genera,among which five potential new species were obtained.The dominant genus was Streptomyces(14.2%).(2)It was found that the crude extracts of nine strains had antibacterial activity against at least one pathogen.The results indicate that there are abundant culturable bacteria and potential new antibiotics resources in the mangrove rhizosphere soils from the Maowei Sea.关键词
红树植物/根际土壤/富集培养/可培养细菌/抑菌活性Key words
mangrove plants/rhizosphere soil/enrichment culture/cultured bacteria/antibacterial activity分类
农业科技引用本文复制引用
李王靖,李蜜,徐淑芬,黎芳婷,易湘茜,刘永宏,高程海..茅尾海红树根际土壤可培养细菌多样性及抑菌活性研究[J].广西植物,2023,43(11):1999-2007,9.基金项目
广西自然科学基金创新团队项目(2020GXNSFGA297002) （2020GXNSFGA297002）
国家自然科学基金(U20A20101 （）
82060640) （）
"八桂学者"专项项目 （）
广西中医药大学桂派中医药传承创新团队项目(2022A007) （2022A007）
广西中医药大学研究生教育创新计划项目(YCSW2022356). （YCSW2022356）
