广西植物2023，Vol.43Issue(11)：2024-2032,9.DOI:10.11931/guihaia.gxzw202208034
药用红树木榄胚轴抗HBV化学成分的研究
Anti-HBV chemical constituents from the hypocotyl of pharmaceutical mangrove Bruguiera gymnorhiza
摘要
Abstract
Bruguiera gymnorhiza hypocotyl is a common marine herbal medicine of the Jing people used in the treatment of hepatitis B.Firstly,MTT and real-time fluorescence quantitative PCR methods were used to determine the anti-HBV activity of different extracted parts of B.gymnorhiza hypocotyl,the chemical constituents of the active extraction parts were isolated and identified using modern chromatographic techniques and spectroscopic methods,and in vitro screening model was employed to test the anti-HBV activity of obtained compounds.The results were as follows:(1)The n-butanol phase of the hypocotyl exhibited anti-HBV activity;(2)A total of 11 compounds were isolated and structurally identified,namely uridine(1),thymidine(2),adenosine(3),oryzalactam(4),n-butyl-O-D-fructopyranoside(5),nortetillapyrone(6),(4R,6S)-4-methoxyl-2,3-dihydroaquilegiolide(7),(4R,6S)-2-dihydromenisdaurilide(8),gallcatechin(9),1-(4-hydroxy-3-methoxy)-phenyl-2-[4-(1,2,3-trihydroxypropyl)-2-methoxy]-phenoxy-1,3-propandiol(10),and(-)-lyoniresinol-9-O-β-D-xylopyrano-side(11),among them,compounds 4,5,7 and 8 were firstly obtained from B.gymnorhiza,and Compound 4 showed anti-HBV activity with an inhibition rate of 23.59%.The study clarify the chemical composition of the anti-HBV of B.gymnorhiza hypocotyl.关键词
木榄胚轴/化学成分/分离纯化/结构鉴定/抗乙肝病毒活性Key words
Bruguiera gymnorhiza hypocotyl/chemical constituents/isolation and purification/structural identification/anti-HBV activity分类
农业科技引用本文复制引用
梁考云,候师师,高程海,刘永宏,易湘茜..药用红树木榄胚轴抗HBV化学成分的研究[J].广西植物,2023,43(11):2024-2032,9.基金项目
国家自然科学基金(81903533) （81903533）
广西自然科学基金项目-创新研究团队项目(2020GXNSFGA297002) （2020GXNSFGA297002）
广西八桂学者专项基金 （）
广西中医药大学桂派中医药传承创新团队项目(2022B005) （2022B005）
广西研究生教育创新计划项目(YCXJ2021133) （YCXJ2021133）
广西中医药大学桂派杏林拔尖人才资助项目(2022C008) （2022C008）
广西中医药大学高层次人才培育创新团队项目(2022A007). （2022A007）
