基础医学与临床2023，Vol.43Issue(12)：1784-1791,8.DOI:10.16352/j.issn.1001-6325.2023.12.1784
GPS2对肝癌细胞系MHCC-97H增殖、迁移与侵袭的调控作用
The role of GPS2 in regulating proliferation,migration and invasion of hepatocellular carcinoma cell line MHCC-97H
摘要
Abstract
Objective To explore the role of G protein pathway suppressor 2(GPS2)in regulating proliferation,migration and invasion in hepatocellular carcinoma cell line MHCC-97H.Methods The expression of GPS2 in hepatocellular carcinoma(HCC)cells were detected using quantitative PCR or Western blot;GPS2 was either over-expressed or knocked down in HCC cells using over-expression vectors or short hairpin RNAs(shRNAs)for GPS2,respectively.Clone formation assay,scratch assay and Transwell assay were used to detect the effects of GPS2 on proliferation,migration and invasion of HCC cells.Results In HCC cells,over-expression of GPS2 was found to inhibit the proliferation,migration and invasion of HCC cells MHCC-97H,while knockdown of GPS2 up-regulated proliferation,migration and invasion of MHCC-97H(P＜0.05).The over-expression of GPS2 in MHCC-97H cells was found to inhibit the expression of cell proliferation,migration and invasion-related factors such as EGFR,MMP1,MMP3 and MMP9,while knockdown of GPS2 upregulated the expression of these factors(P＜0.05).Conclusions GPS2 is a novel HCC regulatory factor that inhibits proliferation,migration and invasion of HCC cells.关键词
肝细胞癌/G蛋白通路抑制因子2(GPS2)/细胞增殖/迁移与侵袭Key words
hepatocellular carcinoma/G protein pathway suppressor 2(GPS2)/cell proliferation/migration and invasion分类
医药卫生引用本文复制引用
刘爱霞,张浩,孙杰,杨丽华,赵晓涛..GPS2对肝癌细胞系MHCC-97H增殖、迁移与侵袭的调控作用[J].基础医学与临床,2023,43(12):1784-1791,8.基金项目
国家科技重大专项(2017ZX10302201) （2017ZX10302201）
