基础医学与临床2023，Vol.43Issue(12)：1814-1821,8.DOI:10.16352/j.issn.1001-6325.2023.12.1814
过表达APMAP减轻阿霉素肾病肾小球足细胞损伤
Over-expression of APMAP alleviates glomerular podocyte injury in adriamycin nephropathy
摘要
Abstract
Objective To investigate the effects of adipocyte plasma membrane-associated protein(APMAP)over-expression on glomerular podocyte injury in adriamycin(ADR)nephropathy.Methods The rat model of adriamy-cin nephropathy was constructed by tail vein injection of adriamycin,the expression of APMAP and NF-κB p65 in renal tissue was measured by immunohistochemistry.A mouse glomerular podocytes MPC-5 cell line with APMAP gene over-expression was constructed,then podocyocytes injury model was induced by 0.5 μmol/L ADR and trea-ted with NF-κB signaling pathway activator CU-T12-9.The proliferation of cells was checked by CCK-8.The activity of lactate dehydrogenase(LDH)was determined by ELISA.The apoptosis of podocytes was determined by flow cytometry.Western blot was used to detect protein expressions of NF-κB p65,p-NF-κB p65 and TNF-α.Results APMAP was expressed in kidney tissue of doxorubicin nephropathy rats at a low level,while NF-κB p65 was significantly high expressed(P＜0.05).Over-expression of APMAP increased proliferation of MPC-5 cells and decreased LDH activity,apoptosis rate,and also down-regulated protein expression of NF-κB p65,P-NF-κB p65 and TNF-α under ADR exposure(P＜0.05).However,combined treatment with CU-T12-9 significantly inhibited the ameliorative effect of APMAP over-expression on the damage of MPC-5 cells exposed to ADR.Conclusions The over-expression of APMAP can inhibit ADR-induced glomerular podocyte injury,and its mechanism might be related to the inhibition of NF-κB signaling pathway.关键词
阿霉素肾病/肾小球足细胞/脂肪细胞膜相关蛋白质(APMAP)/NF-κB信号通路Key words
adriamycin nephropathy/glomerular podocytes/adipocyte plasma membrane-associated protein(APMAP)/NF-κB signa-ling pathway分类
医药卫生引用本文复制引用
吴静漪,范文静,严冬华..过表达APMAP减轻阿霉素肾病肾小球足细胞损伤[J].基础医学与临床,2023,43(12):1814-1821,8.基金项目
苏州市科技计划项目(SYSD2018043) （SYSD2018043）
