水产学报2023，Vol.47Issue(12)：17-36,20.DOI:10.11964/jfc.20210512825
青海湖裸鲤盐碱耐受过程中的渗透、免疫、代谢相关基因筛选和分析
Screening and analysis of the osmotic,immune,metabolic related genes of Gymnocypris przewalskii in saline-alkali tolerance
摘要
Abstract
Gymnocypris przewalskii is an important indigenous economic fish in Qinghai Lake,which plays a key role in the whole Qinghai Lake ecosystem and shows a strong saline-alkali tolerance and adaptability compared with other fishes.In order to explore the important functional genes of G.przewalskii during the process of saline-alkali tolerance,Illumina Hiseq technology was used to define the transcriptome of gill and kidney tissues of G.przewalskii in two areas(Qinghai Lake and the Quanji River).All kinds of clean data reached more than 6.74 Gb,and the percentage of q30 base was more than 94.38%.After quality control and assembly,a total of 541 429 uni-genes were obtained,the average length of N50 was 612 bp.The sequences acquired were blasted against NR,Swiss-Prot,pfam,COG,GO and KEGG databases,finally,107 568 unigenes could be annotated.Differential expression analysis showed that a total of 832 genes were co-expressed in the gills and kidneys in two regions.Gene ontology analysis indicated that differentially expressed genes(DEGs)annotated to binding,cellular process,metabolic process,and single-organism process accounted for a large proportion.Based on KEGG pathway ana-lysis,the pathways related to immunity,metabolism and penetration were enriched.According to the GO annota-tion of DEGs and the enrichment analysis of KEGG signaling pathway,we initially screened 35,34,and 25 genes related to osmotic,immunity,and metabolism of G.przewalskii,respectively.The osmotic related genes included calcium/calmodulin-dependent protein kinase,sodium/potassium transporting ATPase,and mitogen-activated pro-tein kinase,solute carrier family,etc,immune related genes mainly included interleukin,complement,integrin,granzyme B,etc;nitric oxide synthase,1,25-dihydroxyvitamin D(3)24-hydroxylase,cytochrome P450 and elonga-tion factor were related to metabolism of G.przewalskii.Sixteen differentially DEGs were randomly selected for quantitative real-time PCR(qRT-PCR),and the results were consistent with the RNA-seq.The result of this study enriches the basic data of G.przewalskii's omics research,and also provides a reference for further study of G.przewalskii in saline-alkali tolerance.关键词
青海湖裸鲤/转录组测序/盐碱耐受/差异表达基因Key words
Gymnocypris przewalskii/transcriptome sequencing/saline-alkali tolerance/differentially expressed genes分类
生物科学引用本文复制引用
张海琛,马清花,许保可,阿琳林,梁健..青海湖裸鲤盐碱耐受过程中的渗透、免疫、代谢相关基因筛选和分析[J].水产学报,2023,47(12):17-36,20.基金项目
国家自然科学基金(31960741) （31960741）
青海省科技项目(2016-ZJ-940Q) National Natural Science Foundation of China(31960741) （2016-ZJ-940Q）
Science and Technology Project of Qinghai Province(2016-ZJ-940Q) （2016-ZJ-940Q）
