水产学报2023，Vol.47Issue(12)：109-120,12.DOI:10.11964/jfc.20210913080
维生素E对虎龙杂交斑生长、抗氧化及免疫功能的影响
Effects of dietary vitamin E on growth,anti-oxidation and immunity of hybrid grouper juveniles(Epinephelus fuscoguttatus♀×E.lanceolatus♂)
摘要
Abstract
An eight-week feeding trial was carried out to evaluate the influence of vitamin E(VE)on the growth performance,antioxidation and immunity of hybrid grouper juveniles(Epinephelus fuscoguttatus ♀×E.lanceol-atus ♂).Six isoenergetic(340 kcal/100 g dry matter),isoproteinic(51.5%of dry matter)and isolipidic(9%of the dry matter)diets were formulated to contain graded levels of VE(4.1,26.3,40.7,57.1,116.8,209.6 mg/kg,dry matter basis).Triplicate groups of twelve fish[initial average weight of(14.22±0.01)g]were stocked into floating cages and offered their prescribed diet twice daily(8:00 and 16:30)to apparent satiation.After the growth experi-ment,the remained experimental fish from each group were used for a 72 h copper challenge.Results showed that fish fed 57.1 mg/kg VE exhibited higher weight gain percentage(WG)than fish fed 4.1 mg/kg VE.Whereas,condi-tion factor(CF),hepatosomatic index(HSI)intraperitoneal fat(IPF)values remained non-significant among all dietary groups.Whole-body and muscular compositions(moisture,protein and lipid)did not show significant dif-ferences among all dietary groups.Fish fed 209.6 mg/kg VE had higher hepatic VE concentration than fish fed 4.1 mg/kg VE.The total-antioxidant capacity(T-AOC)of liver,serum lysozyme(LZM)and immunoglobulin(IgM)concentrations were increased with the increasing VE levels,reaching a peak value at the 57.1 mg/kg level,and thereafter,it started to decrease as dietary VE level further increased.After the challenge,the survival rate of the experimental fish fed with 40.7,57.1 or 116.8 mg/kg VE diet was higher than that of the experimental fish fed with 4.1 mg/kg VE.The relative expression of nuclear factor E2-related factor 2(Nrf2)in head kidney of fish fed 4.1 mg/kg VE was lower than that of fish fed 40.7,57.1 or 116.8 mg/kg VE.Generally,the analysis of a quadratic broken line model based on WG and T-AOC indicated that the optimal dietary VE requirement of hybrid grouper juvenile was found to be 62.92 mg/kg and 86.25 mg/kg dry matter,respectively.关键词
虎龙杂交斑/维生素E/生长/抗氧化/免疫Key words
hybrid grouper/vitamin E/growth/anti-oxidation/immunity分类
农业科技引用本文复制引用
翟浩云,张璐,蔡亲晓,周智愚,殷浩然,耿丽娜,吴小易..维生素E对虎龙杂交斑生长、抗氧化及免疫功能的影响[J].水产学报,2023,47(12):109-120,12.基金项目
国家自然科学基金(31760760) （31760760）
海南省重点研发计划(ZDYF2018055) National Natural Science Fundation of China(31760760) （ZDYF2018055）
Hainan Key Research and Develop-ment Projects(ZDYF2018055) （ZDYF2018055）
