水产学报2023，Vol.47Issue(12)：135-146,12.DOI:10.11964/jfc.20210913081
植物乳杆菌(LP HMX-3)对仿刺参生长、消化、免疫和肠道菌群的影响
Effects of Lactobacillus plantarum(LP HMX-3)on growth,digestion,immunity and intestinal flora of Apostichopus japonicus
摘要
Abstract
Sea cucumber(Apostichopus japonicus)is one of the most important economic species of mariculture in north China,but the frequent outbreaks of various diseases such as skin ulceration syndrome hindered the sustain-able development of A.japonicus aquaculture.As an important part of disease prevention and control,probiotics will hopefully replace antibiotics and be widely used in aquaculture industry.This experiment was conducted to investigate the effects of Lactobacillus plantarum LP HMX-3 on growth performance,digestive enzyme activity,immune ability and intestinal flora of A.japonicus.A total of 270 A.japonicus with an average initial body weight about(4.22±0.05)g were randomly divided into 3 groups,three replicates for each group,30 A.japonicus for each repetition.The basal diet was used as control group(C group).Two experimental diets were obtained by adding 105 CFU/g(LL group)and 107 CFU/g(LM group)LP HMX-3 to the basal diet.The feeding experiment lasted for 8 weeks.The experimental results show that ①The final body weight,weight gain rate and specific growth rate of juvenile sea cucumber A.japonicus were significantly increased by adding LP HMX-3,and the LL group had better growth index.②Compared with control group,the intestinal amylase activity in LL group and the intestinal lipase activity in LM group were significantly enhanced,while there was no significant difference in intestinal trypsin activity among all groups,but the value of probiotics groups was higher,indicating that LP HMX-3 may improve the digestion and absorption of nutrients in A.japonicus.③Compared with control group,the activities of alkaline phosphatase and catalase were significantly increased in the group of LL and LM,and the acid phosphatase activity was significantly increased in the group of LM.There was no significant difference in super-oxide dismutase activity among all groups.Besides,the mRNA expression levels of Aj-p105 and Aj-catalase in LL and LM groups were significantly increased compared with that in control group,and the mRNA expression level of Aj-C3 in LM group was also significantly higher than that in control group.These results demonstrated that diet-ary with L.plantarum LP HMX-3 supplemented could enhance the immune capacity of A.japonicus.④All alpha indices suggested that LP HMX-3 significantly increased intestinal microbial richness and evenness,but the level of addition had little effect.The Beta diversity analysis indicated that the bacteria communities in probiotic supple-mented groups were quite similar,while different from control group.⑤At the level of phylum,the relative abund-ance of Proteobacteria in control group was significantly higher than any other groups,while that of Firmicutes in LL group increased significantly compared with control group.The relative abundance of Bacteroidota,Cyanobac-teria and Campilobacterota was significantly increased in the group of LL and LM,compared with those in control group.At the genus level,the relative abundance of Lactobacillus,Bifidobacterium,Streptococcus and Clostridium in LL and LM groups was higher than that in C group,indicating that LP HMX-3 significantly increased the abundance of potential probiotics.The results of the study suggested that adding L.plantarum LP HMX-3 to the diet could effectively promote the growth of A.japonicus,improve its digestion and immunity capacity to a cer-tain extent,and actively regulate the intestinal microflora of A.japonicus.In summary,the results of this study confirmed the probiotic effect of L.plantarum LP HMX-3 in A.japonicus,and provided technical support and sci-entific evidence for its application in aquaculture.关键词
仿刺参/植物乳杆菌/生长性能/消化酶/免疫能力/肠道微生物Key words
Apostichopus japonicus/Lactobacillus plantarum/growth performance/digestive enzyme/immune ability/intestinal flora分类
农业科技引用本文复制引用
王梦美,吕成杰,杨顶珑,赵建民..植物乳杆菌(LP HMX-3)对仿刺参生长、消化、免疫和肠道菌群的影响[J].水产学报,2023,47(12):135-146,12.基金项目
中国科学院战略性先导研究计划(XDA23050303) （XDA23050303）
国家自然科学基金(41806196) （41806196）
山东省自然科学基金(ZR2019BD022) （ZR2019BD022）
烟台市科技发展计划项目(2020MSGY066) Strategic Priority Research Program of the Chinese Academy of Sciences(XDA23050303) （2020MSGY066）
National Natural Science Foundation of China(41806196) （41806196）
Natural Science Foundation of Shandong Province(ZR2019BD022) （ZR2019BD022）
Yantai Science and Technology Development Project(2020MSGY066) （2020MSGY066）
