水产学报2023，Vol.47Issue(12)：147-156,10.DOI:10.11964/jfc.20221013728
稀有(鮈)鲫MHC Ⅱβ基因克隆及鲁氏耶尔森菌感染后的表达分析
Cloning of the MHC Ⅱβ gene in Gobiocypris rarus and expression analysis after Yersinia ruckeri infection
摘要
Abstract
In order to explore the molecular characteristics and expression characteristics of the rare minnow MHCⅡβ gene,the 810 bp cDNA sequence of the rare minnow Gobiocypris rarus MHC Ⅱβ was obtained by PCR amp-lification technology,including an open reading frame(ORF)of 759 bp,encoding 252 amino acids(aa).Bioin-formatics analysis showed that there were four conserved cysteine residues and a GXXGXXXGXXXXXXG struc-ture in the amino acid sequence of MHC IIβ,and the consistency with other relative fish was 51.78%-80.56%.The encoded protein molecules included a signal peptide,a MHC IIβ(β-1)domain,a IGc1(β-2)domain and a trans-membrane helical region.Quantitative Real-time PCR(qRT-PCR)results showed that the expression of MHC Ⅱβ gene was the highest in spleen,and higher in head kidney,gill and skin.After artificial infection with pathogenic bacteria,Yersinia ruckeri,the expression of head kidney was significantly up-regulated at 6 h,and liver was signi-ficantly up-regulated at 12 h,skin was significantly up-regulated at 24 h and 48 h,gill was significantly up-regu-lated at 6 to 24 h,and spleen was significantly down-regulated at 6 h,and approached the expression level of the control group at 96 h.The results show that MHC IIβ plays an important role in immune response,which provides reference for further revealing the regulatory mechanism of MHC family immune defense.关键词
稀有(鮈)鲫/MHC Ⅱβ/细菌感染/基因表达Key words
Gobiocypris rarus/MHC Ⅱβ/bacterial infections/gene expression分类
生物科学引用本文复制引用
郑宗林,张进,徐靖琳,刘伟,于文博,方媛林,张景森,段聪,周朝伟..稀有(鮈)鲫MHC Ⅱβ基因克隆及鲁氏耶尔森菌感染后的表达分析[J].水产学报,2023,47(12):147-156,10.基金项目
西南大学博士基金(SWU114025) The Doctoral Fund Project of Southwest University(SWU114025) （SWU114025）
