山东医药2023，Vol.63Issue(34)：1-5,5.DOI:10.3969/j.issn.1002-266X.2023.34.001
口腔鳞状细胞癌组织中miR-138、FOXD1表达与患者临床病理特征和预后的关系
Expression of miR-138 and FOXD1 in oral squamous cell carcinoma tissues and its relationship with pathological parameters and prognosis
摘要
Abstract
Objective To investigate the expression of microRNA-138(miR-138)and forkhead box protein D1(FOXD1)in oral squamous cell carcinoma(OSCC)tissues and its relationship with pathological parameters and progno-sis.Methods Eighty cases of OSCC tissues and corresponding paracancerous tissues from OSCC patients that underwent surgical resection were collected as study subjects,and miR-138 and FOXD1 mRNA expression levels were detected by re-al-time fluorescence quantitative polymerase chain reaction.We analyzed the relationships between miR-138 and FOXD1 mRNA expression in OSCC tissues and clinicopathological features.OSCC tissues were divided into the high and low ex-pression groups according to the mean values of miR-138 and FOXD1 mRNA expression.The binding sites between miR-138 and FOXD1 were predicted by TargetScan.Pearson correlation was used to analyze the correlation between miR-138 and FOXD1 mRNA expression in OSCC tissues,Kaplan-Meier method was used to plot the survival curves of OSCC pa-tients with different miR-138 and FOXD1 mRNA expression,and multifactorial Cox regression was used to analyze the in-fluencing factors for the prognosis of OSCC patients.Results Compared with the paracancerous tissues,miR-138 ex-pression decreased and FOXD1 mRNA expression increased in OSCC tissues(both P<0.05).MiR-138 had a binding site at the 3´ untranslated end of FOXD1 at 2942-2948.Pearson correlation analysis showed that miR-138 was negatively corre-lated with FOXD1 mRNA expression in OSCC tissues(r =-0.621,P<0.001).Significant differences were found in the miR-138 and FOXD1 mRNA expression levels among OSCC tissues with different degrees of differentiation,TNM stage,depth of infiltration,and lymph node metastasis(all P<0.05).The 5-year overall survival rate of 80 patients with OSCC was 55.00%(44/80).Kaplan-Meier survival curve analysis showed that the overall survival rate was higher in the miR-138 high-expression group than in the low-expression group,and the overall survival rate was lower in the FOXD1 mRNA high-expression group than in the low-expression group(both P<0.05).Multifactorial Cox regression analysis showed that poor differentiation,TNM stage III,infiltration depth≥5 cm,lymph node metastasis,and FOXD1 mRNA≥2.18 were in-dependent risk factors for death in patients with OSCC,and miR-138≥0.78 was an independent protective factor(all P<0.05).Conclusion MiR-138 is low expressed and FOXD1 mRNA is highly expressed in OSCC tissues,which are asso-ciated with the degree of differentiation,TNM stage,depth of infiltration,lymph node metastasis,and prognosis,and may be markers for diagnosis and prognosis of OSCC patients.关键词
口腔鳞状细胞癌/微小核糖核酸-138/叉头框蛋白D1/病理特征/预后Key words
oral squamous cell carcinoma/microRNA-138/forkhead box protein D1/pathological features/prog-nosis分类
医药卫生引用本文复制引用
梁伟腾,王丽娟,刘凡琪,李玲玲,咸钧,牟戎..口腔鳞状细胞癌组织中miR-138、FOXD1表达与患者临床病理特征和预后的关系[J].山东医药,2023,63(34):1-5,5.基金项目
天津市卫生健康科技项目(ZC20044). （ZC20044）
