山东医药2023，Vol.63Issue(34)：38-42,5.DOI:10.3969/j.issn.1002-266X.2023.34.008
康柏西普结膜下注射对兔小梁切除术后滤过通道内瘢痕形成的影响
Effect of Conbercept on scar formation in rabbit filter channel after trabeculectomy
摘要
Abstract
Objective To investigate the effect of Conbercept on fiber scar formation in rabbit filter channel after tra-beculectomy.Methods Forty healthy New Zealand white rabbits were randomly divided into four groups:trabeculecto-my combined with mitomycin group(group A),trabeculectomy combined with Conbercept injection group(group B),trab-eculectomy combined with mitomycin and Conbercept injection group(group C),trabeculectomy combined with normal sa-line injection group(group D),with 10 rabbits in each group.Standard trabeculectomy was performed on the right eyes of rabbits.The intraocular pressure(IOP)was measured before and after the operation,and the morphology and complications were observed by slit-lamp microscope.At 28 days after surgery,the operative eye was removed and the tissues of the follicle area were clipped.HE staining was used to observe the histopathologic changes of the filter pas-sage,Masson staining was used to observe the collagen deposition and fibrosis degree of the filter passage tissues,and immunohistochemical staining was used to observe the expression of CD31 in the filter passage tissues.Results The IOP in each group decreased to the lowest on the 3rd day after surgery,gradually increased on the 7th day after surgery,and almost completely recovered to the preoperative level on the 28th day after surgery.At 7 days after surgery,functional follicles were formed in the operative area of groups A,B and C,while conjunctival vessels on the follicle surface of group D were dense,with obvious vascularization and flat follicle area.Among them,the height of follicles in the group A in-creased and the surface blood vessels were sparse.In the group B,the diffusion of follicles increased,and there was no ob-vious congestion on the follicle surface.In the group C,the diffusion of follicles increased,and there was no obvious con-gestion on the surface of follicles and conjunctiva.As time went on,to 28 days after surgery,the conjunctival congestion gradually decreased and faded away,and the filtration vesicles were limited and flattened.At 28 days after operation,the histopathological features of the eyes in the groups A and B were similar,showing loose collagen fiber arrangement and lo-cal thickened epithelial tissues,accompanied by dot infiltration of lymphocytes.More epithelial cell degeneration,cell swelling and cytoplasmic vacuolation were observed in the groups A and C,while no such pathological changes were ob-served in the group B.At 28 days after surgery,the proportion of collagen fibers in the filter aisle in the groups A,B and C was lower than that in the group D(all P<0.05),and the proportion of collagen fibers in the filter aisle in the group B was lower than that in the groups A and C(both P<0.05).The number of microvessels in the group B was significantly smaller than that in the groups A,C and D(all P<0.05).Conclusion Conbercept can inhibit the formation of neovasculariza-tion and scarring in the rabbit filter passage after trabeculectomy,and help maintain the shape of the filter vesicle,so as to effectively improve the success rate of glaucoma trabeculectomy.关键词
青光眼/小梁切除术/康柏西普/瘢痕/新生血管/大白兔Key words
glaucoma/trabeculectomy/Conbercept/scar/angiogenesis/big white rabbit分类
医药卫生引用本文复制引用
孙中华,刘翠娟,张艳,纪珍,刘伟,王建荣,孙嬿,刘明坤,张苗苗..康柏西普结膜下注射对兔小梁切除术后滤过通道内瘢痕形成的影响[J].山东医药,2023,63(34):38-42,5.基金项目
济南市科技计划临床医学科技创新项目(202019067). （202019067）
