摘要
Abstract
Objective To investigate the effects and mechanism of anlotinib on the migration and invasion of human gastric cancer cells.Methods Human gastric cancer AGS cells were cultured in vitro,and the AGS cells of the 3rd gen-eration in the logarithmic growth phase were selected.They were divided into the control group(no intervention),experi-mental group(2 µmol/L anlotinib group,4 µmol/L anlotinib group,and 8 µmol/L anlotinib group)and inhibitor group[4 µmol/L anlotinib+10 µmol/L transforming growth factor-β1(TGF-β1)/Smads signaling pathway inhibitor LY2109761],which were all treated for 24 h.In order to avoid interference with the experiment of too much cell death,in the inhibitor group,we selected 4 µmol/L anlotinib as the optimal concentration and added 10 µmol/L TGF-β1/Smads pathway inhibi-tor LY2109761 for follow-up study.Cell viability,cell morphology,migration,invasion,and expression levels of epitheli-al mesenchymal transformation(EMT)and transforming growth faction-β1(TGF-β1)/Smads signaling pathways were ana-lyzed by CCK-8,inverted microscopy,Transwell chamber,and Western blotting.Results Compared with the control group,the cell viability of the 4 and 8 µmol/L anlotinib groups decreased(both P<0.05).In the experimental group,the growth of AGS cells was inhibited,and the migration and invasion ability and the expression of TGF-β1,p-Smad3,N-cad-herin,Vimentin and fibronectin(FN)decreased(all P<0.05),and the expression of Smad7 and E-cadherin protein in-creased,with a dose-dependent manner(all P<0.05).Compared with the 4 µmol/L anlotinib group,the cell growth was inhibited,the cell migration and invasion abilities and the protein expression levels of TGF-β1,p-Smad3,N-cadherin,Vimentin and FN further decreased in the inhibitor group(all P<0.05),while the protein expression levels of Smad7 and E-cadherin further increased(both P<0.05).Conclusion Anlotinib can inhibit the migration,invasion and EMT pro-cess of AGS cells in human gastric cancer by mediating TGF-β1/Smads pathway signal transduction.关键词
胃癌/安罗替尼/转化生长因子-β1/Smads信号通路/细胞迁移/细胞侵袭Key words
gastric carcinoma/anlotinib/transforming growth factor-β1/Smads signaling pathway/cell migration/cell invasion分类
医药卫生
