山西农业大学学报（自然科学版）2023，Vol.43Issue(6)：34-43,10.DOI:10.13842/j.cnki.issn1671-8151.202308019
高粱抗蚜虫种质评价及抗性机理解析
Evaluation of sorghum germplasm for aphid-resistant and analysis of resistance mechanism
摘要
Abstract
[Objective]The aim of this study was to identify sorghum aphid-resistant germplasm resources through field inocula-tion.The goal was to reveal the metabolites,metabolic pathways,and functional differences of aphid-resistant materials through physiological metabolomics analysis.Additionally,the research sought to enhance sorghum parental materials through breeding practice to facilitate the development of sorghum variety with resistance to aphid.[Method]In the summer of 2022,22 breeding materials were field-inoculated and evaluated,resulting in the identification of one highly resistant and one highly sus-ceptible material.During the winter jointing stage of 2022,samples were collected for physiological metabolomics determina-tion,three days post-inoculation.[Result]Significant differences were observed in the susceptibility of the 22 materials after in-oculation.Among these,three high-resistant materials and two high-susceptible materials were identified.Specifically,the high-susceptible maintainer line,LB36(aphid ratio 1.78),and the high-resistant maintainer line,LB34(aphid ratio 0.12),were selected for further investigation.The results of physiological metabolomics analysis revealed 140 differential metabolites in the comparison of the resistant and susceptible materials(K1 vs G1)before inoculation,and 181 differential metabolites after inoculation(K2 vs G2).Subsequent comparisons highlighted 17 significantly up-regulated and 24 significantly down-regulated differential metabolites in the comparison of K1 vs K2,and 51 significantly up-regulated and 42 significantly down-regulated dif-ferential metabolites in the highly susceptible materials(G1 vs G2).Both resistant and susceptible materials were enriched in two KEGG metabolic pathways related to of phenylpropanoid biosynthesis and phenylpropanoid compound biosynthesis.In ad-dition,the differential metabolite L-glutamine exhibited an up-regulation trend in high-resistant materials,contrasting with its down-regulation after inoculation in high-susceptible materials.[Conclusion]Among the 22 parental lines,three were identified as high-resistant materials,including one maintainer line(LB34)and two restorer lines(NK1 and LNR-4).Two materials ex-hibited high susceptibility,with one maintainer line(LB36)and one restorer line(LTR168).The enrichment of LB34 in phen-ylpropanoid biosynthesis and phenylpropanoid compound biosynthesis pathways,reflected the crucial role of these pathways in plant growth and resistance to biotic or abiotic stress.The differential metabolites L-glutamine demonstrated significant contri-bution by promoting energy metabolism,enhancing the antioxidant enzyme activity of plants,ultimately leading to resistance against aphid infestation.关键词
高粱/蚜虫/种质/生理代谢Key words
Sorghum/Aphids/Germplasm/Physiological metabolism分类
农业科技引用本文复制引用
王佳旭,李月婷,王艳秋,卢峰,张飞,王金阳,朱凯..高粱抗蚜虫种质评价及抗性机理解析[J].山西农业大学学报（自然科学版）,2023,43(6):34-43,10.基金项目
辽宁省藏粮于技重大专项(2023JH1/10200001) （2023JH1/10200001）
国家现代农业产业技术体系(CARS-06-14.5-A22) （CARS-06-14.5-A22）
辽宁省农业科学院院长基金(2022QN2317) （2022QN2317）
