山西农业大学学报（自然科学版）2023，Vol.43Issue(6)：99-108,10.DOI:10.13842/j.cnki.issn1671-8151.202307017
不同状态下华北落叶松枝条化学成分分析
Chemical composition analysis of branches of Larix principis-rupprechtii Mayr under different condi-tions
摘要
Abstract
[Objective]The aim of the study was to clarify the chemical substances emitted by Larix principis-rupprechtii Mayr,and to provide a reference for identifying chemicals that might attract or repel insects of L.principis-rupprechtii.[Methods]The dynamic headspace adsorption method was used to extract volatiles from branches of L.principis-rupprechtii under three different conditions,including healthy,mechanically damaged,and insect damaged.The volatiles were identified and analyzed using gas chromatography-mass spectrometry(GC-MS)technology.[Results]The results showed that a total of 59 volatile compounds were identified under three conditions.The branches of L.principis-rupprechtii released 52,53 and 53 kinds of volatiles under three treatment conditions of health,pest,and mechanical damage,respectively.These compounds en-compassed ten categories:aromatic hydrocarbons,ketones,alcohols,terpenes,esters,aldehydes,acids,phenols,alkanes,and ethers.Four categories showed higher levels,with alkanes being the most abundant,followed by ketones,terpenes,and aldehydes,while the remaining six categories exhibited lower levels.There were three unique volatile compounds under the healthy condition,such as heptane,1,3,5-triethylbenzene,and n-heptacosane.Under insect-infested conditions,three unique volatiles were detected,including 3-carene,n-hexane,and n-octadecane.No specific volatiles were detected under the mechan-ical damage condition.Forty-five compounds were shared across all three conditions,with four compounds exhibiting higher contents:4-ethylbenzaldehyde,3-ethylacetophenone,4-ethylacetophenone,and β-pinene and(+)-pinene.Five compounds displayed significant differences,namely(+)-α-pinene,β-pinene,cetylaldehyde,2,2'-methylene bis-(4-methyl-6-tert-butyl-phenol),and 2,6,10-trimethyl-dodecane.Among the major components,(+)-α-pinene,β-pinene,and 2,2'-methylene bis-(4-methyl-6-tert-butylphenol)showed significantly higher concentrations.Notable,the(+)-α-pinene and β-pinene levels under mechanically injury and insect infestation conditions were significantly higher than those under the healthy condition(P＜0.05).[Conclusion]The branches of L.principis-rupprechtii exhibited variations in alkanes and terpenes under three different treat-ments as health,insect damaged,and mechanically damaged states.The relative content of alkanes in the mechanical damaged state was approximately 10％higher than that in health and insect damaged state,while the terpene content was about 15％low-er.关键词
华北落叶松/挥发物/动态顶空吸附/气相色谱-质谱联用Key words
Larix principis-rupprechtii Mayr/Volatiles/Dynamic headspace adsorption/Gas chromatography-mass spectrom-etry分类
农业科技引用本文复制引用
白秀英,张平,杨美红,赵祥..不同状态下华北落叶松枝条化学成分分析[J].山西农业大学学报（自然科学版）,2023,43(6):99-108,10.基金项目
山西省专利推广实施资助专项(20200728) （20200728）
山西农业大学引进人才博士科研启动项目(412576) （412576）
