特产研究2023，Vol.45Issue(6)：37-45,52,10.DOI:10.16720/j.cnki.tcyj.2023.219
基于网络药理学和实验验证探讨赤芍治疗胆汁淤积性肝炎的分子机制
Study on the Effective Components and Molecular Mechanism of Radix Paeoniae Rubra in the Treatment of Cholestatic Liver Disease based on Network Pharmacology
摘要
Abstract
To investigate the molecular mechanism of Paeoniae Rubra Radix(PRR)in the treatment of Cholestatic Hepatitis(CH).The chemi-cal components of PRR were retrieved by TCMSP database and the underlying active ingredients was screened by the OB and DL.The potential targets of active ingredients of PRR were predicted by Pharmmapper and Swiss Target Prediction database.The related targets of CH were ob-tained by DrugBank,TTD and Genecards database.The common targets of active ingredients and CH was acquired by using Venny2.1.0 online platform.PPI network was constructed through STRING database and was visualized by Cytoscape 3.9.0.GO function enrichment analysis and KEGG pathway enrichment analysis were carried out through DAVID database.'Components-targets-pathways'network was constructed through Cytoscape 3.9.0 software.ANIT was used to establish rat model of cholestasis,and Paeoniae Rubra Radix extract(PRRE)was administered for intervention,the protein expression in the liver tissue of the rats in each group was investigated by immuno-histochemistry(IHC).28 potential active ingredients.34 intersection targets of PRR in the treatment of CH were screened by Venny2.1.0 online platform.6 key targets were screened according to the degree value.The results of IHC showed that the expressions of EGFR,VEG-FA,and p38MAPK in the PPRE administration group were significantly decreased compared with the model group,and the difference was statistically significant(P＜0.05).PRR may play a role in the treatment of CH by intervening EGFR,VEGFA,p38MAPK and other targets through paeoniflorin and paeoniflorin,thereby regulating MAPK and other signaling pathways.关键词
网络药理学/赤芍/胆汁淤积性肝炎/分子机制/MAPK信号通路Key words
network pharmacology/Paeoniae Rubra Radix/cholestatic hepatitis/molecular mechanism/MAPK signaling分类
医药卫生引用本文复制引用
王文祥,唐晓翠,李宁,杨策,陈芸彤,陈春宇..基于网络药理学和实验验证探讨赤芍治疗胆汁淤积性肝炎的分子机制[J].特产研究,2023,45(6):37-45,52,10.基金项目
重庆市教育委员会科学技术研究计划重点项目(KJZD-K202202701) （KJZD-K202202701）
重庆三峡医药高等专科学校重大项目(XJ2021000301) （XJ2021000301）
