新疆农业科学2023，Vol.60Issue(11)：2618-2626,9.DOI:10.6048/j.issn.1001-4330.2023.11.003
高温胁迫下陆地棉转录组差异性分析
Analysis of the difference of transcription groups of upland cotton under heat stress
摘要
Abstract
[Objective]To explore the response of key genes to heat stress and compare the differences of gene expression.[Methods]RNA-Seq was used to sequence and analyze the transcriptome of YZ1 unde2r normal(26℃)and high temperature stress(42℃).[Results]Gohir.A08G10410A total of 383 up-regulated genes and 234 down regulated genes were identified in WN4/WH4,WN12/WH12,WN24/WH24 and WN48/WH48.According to GO function,the differential genes were mainly enriched into 20 categories,namely,up-regulated differential genes and down-regulated differential genes.According to KEGG function analysis,the differential genes were significantly enriched in flavonoid biosynthesis pathway,phenylalanine metabolism,circadian rhythm-plant and endoplasmic reticulum processing and other pathways.39 transcrip-tion factors were identified,mainly belonging to WRKY and MYB families;Combined with gene annotation,it was found that the expression levels of these two genes were significantly different in the whole period.Gohir.A08G104100 was the main gene of heat stress,which was crucial to the heat tolerance of plants,while Gohir.D08G033300 was a limiting enzyme for plastid isoprene biosynthesis and was critical for chloroplast develop-ment.[Conclusion]Through comparative transcriptome analysis,the difference of gene transcription expres-sion between upland cotton under high temperature stress and normal temperature is compared.关键词
RNA-Seq/陆地棉/高温胁迫/差异表达/代谢通路Key words
RNA-Seq/Gossypium hirsutum/heat stress/differential expression/metabolic pathway分类
农业科技引用本文复制引用
王辉,董永梅,郭伟锋,曹新川,郭金成,谢宗铭,何良荣..高温胁迫下陆地棉转录组差异性分析[J].新疆农业科学,2023,60(11):2618-2626,9.基金项目
新疆生产建设兵团农业科技创新工程专项(NCG202224) （NCG202224）
国家自然科学基金项目"GhCIPK6调控棉花耐热胁迫机理研究"(31550010)Xinjiang Production and Construction Corps Agricultural Science and Technology Innovation Project(NCG202224) （31550010）
National Natural Science Foundation of China"Regulation mechanism of heat stress is studied in GhCIPK6 in cotton"(31550010) （31550010）
