新疆农业科学2023，Vol.60Issue(11)：2627-2637,11.DOI:10.6048/j.issn.1001-4330.2023.11.004
陆地棉TCP家族基因鉴定及组织表达分析
Identification of TCP family and analysis of tissue expression in upland cotton
摘要
Abstract
[Objective]Identification and analysis of TCP family genes in upland cotton can lay a founda-tion for studying the biological functions of TCP genes.[Methods]Based on the newly assembled upland cot-ton TM-1 reference genome in 2019,the TCP family HMM model file was obtained from Pfam website,the upland cotton TCP gene was identified using Hmmer website,and the upland cotton TCP genome protein se-quence was obtained from Cotton FGD website.MapInspect software was used for chromosome location,MEGA 7.0 was used for multiple sequence alignment cluster analysis,MEME website was used for motif prediction,TBtools software was used for gene structure identification and tissue-specific expression analysis of upland cotton.[Results]Sixty-three TCP genes of upland cotton were identified from the whole genome level,inclu-ding 39 subclasses of Class Ⅰ and 24 subclasses of Class Ⅱ.The Class Ⅱ subfamilies included 17 CIN subclas-ses and 7 CYC/TB1 subclasses.The 63 genes were distributed on 22 chromosomes of upland cotton,including 33 genes in group A and 30 genes in Group D.All TCP proteins contained TCP domains.The gene structure analysis results showed that the structures and lengths of exon and introns were similar in the same subfamily.12 of the TCP family genes were preferentially expressed in fibrous tissue,32 in flower organs,and 16 in roots,stems and leaves.[Conclusion]In this study,TCP genes related to flowering regulation and fiber de-velopment in upland cotton are identified and obtained by bioinformatics method.关键词
陆地棉/TCP基因/基因家族鉴定/生物信息学/特异性表达Key words
upland cotton/TCP gene/family identification/bioinformatics/specific expression分类
农业科技引用本文复制引用
胡文冉,赵准,邵武奎,黄全生..陆地棉TCP家族基因鉴定及组织表达分析[J].新疆农业科学,2023,60(11):2627-2637,11.基金项目
新疆维吾尔自治区自然科学基金面上项目(2022D01A88) （2022D01A88）
新疆维吾尔自治区重点实验室开放课题(2021D04002)The General Project of Natural Science Foundation of Xinjiang Uygur Autonomous Region(2022D01A88) （2021D04002）
The Open Project of the Key Laboratory of Xinjiang Uygur Autonomous Region(2021D04002) （2021D04002）
