畜牧与兽医2023，Vol.55Issue(12)：1-7,7.
鸡内皮素3(EDN3)基因启动子区序列生物信息学分析
Bioinformatics analysis of promoter region sequence of EDN3 gene in chicken
摘要
Abstract
In order to analyze the structural characteristics of the promoter region sequence of the EDN3 gene in chickens and explore the transcriptional regulation mechanism of the sequence.Based on the 2 052 bp nucleotide sequence of 5'UTR and exon 1 of the chicken EDN3 gene registered in the NCBI database,the core promoter region,cis-acting elements,transcription factor binding sites and CpG island of the sequence were predicted by means of a variety of online softwares.The similarity of the EDN3 gene promoter sequence between chicken and other species was compared,and phylogenetic tree construction was carried out using DNASTAR Lasergene 17.3 and MEGA 5.0.The results showed that the 592-2 000 bp upstream of the initiaton codon of the chicken EDN3 gene was a possible candidate core promoter region,and two potential transcription initiation sites T and A were found at 633 bp and 1 547 bp of the 5'UTR.There were two CAAT-boxes,three GC-boxes,six TATA-boxes,nine E-boxes and three CpG island regions in the promoter region of the EDN3 gene.The sequence also con-tained some transcription factor binding sites,such as Sp1,C/EBPα and NF-1.The similarity of the promoter region sequence for the EDN3 gene between chickens and ring-necked pheasants,quails,rock ptarmigans,ruddy ducks,crested ducks,goats,sheep,cattle,pigs,hu-mans was 38.8％-90.3％.The phylogenetic tree revealed that chickens were the most closely related to quails,and the farthest related were pigs.In this study,bioinformatics software was used to predict and analyze the promoter sequence of the EDN3 gene in chickens,which pro-vided theoretical reference and basis for further revealing the transcriptional regulation mechanism of the EDN3 gene in pigmentation trait of chickens.关键词
鸡/内皮素3基因/启动子区/转录因子/CpG岛Key words
chicken/EDN3 gene/promoter region/transcription factor/CpG island分类
农业科技引用本文复制引用
宋兴超,安清明,张晓东,孟金柱,赵园园,吴震洋..鸡内皮素3(EDN3)基因启动子区序列生物信息学分析[J].畜牧与兽医,2023,55(12):1-7,7.基金项目
贵州省科技计划项目(黔科合基础-ZK[2022]一般559) （黔科合基础-ZK[2022]一般559）
贵州省重点实验室项目(黔科合平台人才[2020]2003号) （黔科合平台人才[2020]2003号）
