畜牧与兽医2023，Vol.55Issue(12)：8-12,5.
羔羊不同超排方法的筛选及其对卵母细胞体外成熟的影响
Screening of superovulation methods in lambs and their effects on oocyte maturation in vitro
摘要
Abstract
This study was to compare the effects of different superovulation protocols on lamb supernumeraries and on their oocyte matura-tion in vitro and to screen the best superovulation protocol for the young animals.The experiment was conducted on 1-2 month old sheep lambs which were divided into three groups,two of which,the scheme Ⅰ and scheme Ⅱ groups,were injected with hormones as the experi-mental superovulation groups and one of which was the control group without hormone injection,so as to determine the effects of different su-perovulation methods on ovarian responsiveness,ovarian size and oocyte maturation in vitro in sheep lambs.The results showed that the ovu-lation protocol Ⅱ groups treated with 6x12 h intervals and a total dose of 180 U FSH was more effective than the ovulation protocol Ⅰ group with 4x12 h intervals and a total dose of 200 U FSH.The oocyte maturation rate was 45.4％in the superovulation Ⅱ group,as compared to 32.2％in the superovulation Ⅰ group.To conclude,screening of a superovulation protocol with 6x12 h intervals,a total dose of 180 U FSH and 330 U PMSG injected intramuscularly at the same time as the first FSH injection was the best superovulation protocol for the young animals,which laid a theoretical foundation for sheep lambs in expanding the source of eggs needed for in vitro fertilization,in shortening the generation interval,in producing a large number of excellent offspring and in speeding up the breeding process.关键词
绵羔羊/体外成熟/卵母细胞/超数排卵Key words
lamb/in vitro maturation/oocyte/superovulation分类
农业科技引用本文复制引用
刘昱成,王晶晶,张振良,倪建宏,刘长彬,傅祥伟,石国庆,万鹏程,崔炳灿..羔羊不同超排方法的筛选及其对卵母细胞体外成熟的影响[J].畜牧与兽医,2023,55(12):8-12,5.基金项目
国家绒毛用羊产业技术体系(CARS-39-07),国家重点研发计划(2020YFD1300900) （CARS-39-07）
兵团科技特派员创新创业计划项目(2022CB026) （2022CB026）
新疆农垦科学院院级科研项目(2023YJ010) （2023YJ010）
八师创新环境建设和能力提升计划项目(2023RC07) （2023RC07）
