畜牧与兽医2023，Vol.55Issue(12)：62-67,6.
应用Cephodex微载体培养DF-1细胞增殖水貂源犬瘟热病毒的技术研究
Investigation into the technology of culturing DF-1 to proliferate canine distemper virus isolated from mink with Cephodex micro-carrier
摘要
Abstract
In order to establish a large-scale suspension culture technology of canine distemper virus(CDV)isolated from mink and realize its high-density cell growth and efficient virus proliferation,Cephodex was used to culture DF-1 cells by suspension to proliferate CDV3.In the whole process,shake flask culture was adopted,and optimal culture conditions were established by optimizing the key technical condi-tions such as virus culture temperature and virus harvest time.The results showed that,after being cultured at 37 ℃ for 72 hours,and the DF-1 cells were inoculated with CDV3.Then,these cells were infected by the CDV3 and continued to be cultured at 35 ℃ for 72 hours,and finally the viral titer reached 105.0 TCID50 per 0.1 mL.Therefore,a technology of CDV micro-carrier suspension culture was established here,which laid an important technical foundation for high-efficiency mink canine distemper vaccine production.关键词
犬瘟热病毒/微载体/悬浮培养/DF-1细胞/疫苗Key words
canine distemper virus/micro-carrier/suspension culture/DF-1 cells/vaccine分类
农业科技引用本文复制引用
梅建国,王建军,庄金秋,任强,莫玲,王艳,李峰,郭时金,付石军,赵蕾..应用Cephodex微载体培养DF-1细胞增殖水貂源犬瘟热病毒的技术研究[J].畜牧与兽医,2023,55(12):62-67,6.基金项目
山东省生猪产业技术体系(SDAIT-08-13) （SDAIT-08-13）
