畜牧与兽医2023，Vol.55Issue(12)：75-84,10.
猪源ST3型多杀性巴氏杆菌(血清A型)的鉴定及致病性研究
Identification and pathogenicity of the type ST3 Pasteurella multocida strain isolated from pig
摘要
Abstract
The aim of this study was to identify the pathogenicity of dead pigs suffering from respiratory diseases in Liupanshui Pig Farm.Oral and nasal swabs and lung tissues of sick pigs were collected afflicted pigs for pathogen isolation,identification and pathogenicity analy-sis.The biological characteristics of the pathogenic bacteria were investigated using physiological and biochemical methods,16S rDNA gene sequence analysis,MLST typing,virulence gene and drug resistance gene detection and artificial infection test.The results showed that the isolated strain was Gram-negative bacillus,which was identified by PCR and 16S rDNA sequencing as Pasteurella multocide and it was named PM0712.It was found that the isolated strain PM0712 was swine type A Pasteurella multocida.MLST analysis showed that PM0712 belonged to the ST3 type.The isolated strain carried 17 virulence genes and 4 resistance genes,and it possessed moderate biofilm formation ability,with a median lethal dose of 1.6 × 101 CFU/mL in mice.PM0712 was sensitive to antibiotics such as ciprofloxacin and norfloxacin,and traditional Chinese medicines Coptis chinensis and Fructus Mume.The present results provided a reference for prevention and control of swine type ST3 Pasteurella multocida infection and for research on the pathogenic mechanism of the infection.关键词
多杀性巴氏杆菌/分离鉴定/MLST/致病性Key words
Pasteurella multocida/isolation and identification/MLST/pathogenicity分类
农业科技引用本文复制引用
赵苗苗,孙殿钢,项维,王玲玲,刘峰,何斌,李丰阳,雷连成,张付贤..猪源ST3型多杀性巴氏杆菌(血清A型)的鉴定及致病性研究[J].畜牧与兽医,2023,55(12):75-84,10.基金项目
国家重点研发计划项目(2021YFD1800405) （2021YFD1800405）
国家自然科学基金面上项目(32072823) （32072823）
