畜牧与兽医2023，Vol.55Issue(12)：98-104,7.
弓形虫LAMP荧光检测方法的建立与应用
Establishment and application of a LAMP fluorescence method for detection of Toxoplasma gondii
杜鹃 1王林 1吴迪 1张玮 1程敏姮 1李蕊 1李刚 1陈会玲 1王天梓 2苗东影1
作者信息
- 1. 北京市动物疫病预防控制中心,北京 102629
- 2. 北京市大兴区农业农村局检测检疫和疫病防控中心,北京 102629
- 折叠
摘要
Abstract
In order to establish a loop mediated isothermal amplification(LAMP)fluorescence method for detecting Toxoplasma gondii,two sets of primers were designed and synthesized using highly repetitive conservative sequences of Toxoplasma gondii as templates.The prim-er sets were compared and reaction conditions were optimized to determine specificity and sensitivity of the detection mehtod.The results showed that the sensitivity of the established method for detection of Toxoplasma gondii within 62 ℃ and 50 min was 1 copies/uL,which was higher than that of the standard method and superior to a commercially available real-time fluorescence quantitative PCR detection kit.There was no cross reaction with Schistosoma japonicum,Toxocara felis,Babesia canis,Neospora canis,and Leptospira.Then,the LAMP fluores-cence detection method was used to detect 200 clinical samples of Toxoplasma gondii with known background information,and 10 positive samples and 190 negative samples were detected.The detection results were consistent with those of the standard method and the fluorescence quantitative PCR detection.The innovative points of this method are its rapidity,high sensitivity,strong specificity,and wide application range of instruments and equipment,which are suitable for promotion and application in veterinary laboratories and animal hospitals.关键词
环介导等温扩增/弓形虫/检测Key words
LAMP/Toxoplasmosis/rapid detection分类
农业科技引用本文复制引用
杜鹃,王林,吴迪,张玮,程敏姮,李蕊,李刚,陈会玲,王天梓,苗东影..弓形虫LAMP荧光检测方法的建立与应用[J].畜牧与兽医,2023,55(12):98-104,7.
