眼科新进展2023，Vol.43Issue(12)：952-957,6.DOI:10.13389/j.cnki.rao.2023.0189
miR-1-3p调控ANXA2表达对高糖诱导的视网膜微血管内皮细胞新生血管生成的影响
Effect of micro ribonucleic acid-1-3p on high glucose-induced neovasculariza-tion of retinal microvascular endothelial cells through regulation of Annexin A2 expression
摘要
Abstract
Objective To investigate the possible action mechanism of the micro ribonucleic acid-1-3p(miR-1-3p)/Annexin A2(AnxA2)molecular axis in high glucose(HG)-induced neovascularization of human retinal microvascular en-dothelial cells(HRMECs).Methods A cell injury model was established by culturing HRMECs in vitro and treating them with HG.The HRMECs were divided into the Con group(DMEM medium containing fetal bovine serum in volume fraction of 10％),HG group(cultured in 25 mmol·L-1 D-glucose),HG+miR-NC group(transfected with miR-NC),HG+miR-1-3p group(transfected with miR-1-3p mimics),HG+sh-NC group(transfected with sh-NC),HG+sh-AnxA2 group(transfect-ed with sh-AnxA2),HG+miR-1-3p+pcDNA group(transfected with miR-1-3p mimics+pcDNA),and HG+miR-1-3p+pcDNA-AnxA2 group(transfected with miR-1-3p mimics+pcDNA-AnxA2).After 48 h of transfection,cells were collected and cultured in 25 mmol·L-1 D-glucose medium for 24 h.Cell viability and number of migrating cells were detected using MTT and Transwell chamber experiments,respectively.The number of lumen formations was detected by the lumen forma-tion experiment.The dual luciferase reporter assay was adopted to detect the targeting relationship between miR-1-3p and AnxA2.Western blot was used to detect the protein levels of vascular endothelial growth factor(VEGF)and matrix metal-loproteinase-2(MMP-2).Results Compared with the Con group,the expression level of miR-1-3p in the HG group de-creased,while the levels of AnxA2 messenger ribonucleic acid(mRNA)and protein increased,with statistically significant differences(all P＜0.05).Compared with the Con group,the HG group showed an increase in cell viability,number of mi-grating cells,lumen formation and protein levels of VEGF and MMP-2,with statistically significant differences(all P＜0.05).Compared with the HG+miR-NC group,the HG+miR-1-3p group showed a decrease in cell viability,number of migrating cells,lumen formation and protein levels of VEGF and MMP-2,with statistically significant differences(all P＜0.05).Compared with the HG+sh-NC group,the HG+sh-AnxA2 group showed a decrease in cell viability,number of mi-grating cells,lumen formation and protein levels of VEGF and MMP-2,with statistically significant differences(all P＜0.05).Compared with the HG+miR-1-3p+pcDNA group,the HG+miR-1-3p+pcDNA-AnxA2 group showed an increase in cell viability,number of migrating cells,lumen formation and protein levels of VEGF and MMP-2,with statistically signifi-cant differences(all P＜0.05).Conclusion Overexpression of miR-1-3p can inhibit proliferation,migration and neovas-cularization of HRMECs by targetedly regulating AnxA2 expression.关键词
微小RNA-1-3p/膜联蛋白A2/高糖/视网膜微血管内皮细胞/新生血管/细胞增殖/细胞迁移Key words
micro ribonucleic acid-1-3p/Annexin A2/high glucose/retinal microvascular endothelial cells/neovas-cularization/cell proliferation/cell migration分类
医药卫生引用本文复制引用
巨朝娟,许寅聪,李康宁,石笑楠,熊朝晖,戴明明..miR-1-3p调控ANXA2表达对高糖诱导的视网膜微血管内皮细胞新生血管生成的影响[J].眼科新进展,2023,43(12):952-957,6.基金项目
河北省重点科技研究计划(编号:20210842) （编号:20210842）
河北省卫生健康委员会项目(编号:20191762) （编号:20191762）
