中国农业科技导报2023，Vol.25Issue(12)：67-84,18.DOI:10.13304/j.nykjdb.2023.0132
发情周期对香猪卵巢生物钟相关基因表达的影响
Effects of Estrus Cycle on Expression of Ovarian Biological Clock-related Genes of Xiang Pig
摘要
Abstract
The biological clock plays a critical role in the reproductive system of various living organisms like swine.To examine the effects of estrus cycle on the expression of ovarian biological clock-related gene in Xiang pig,this study analyzed the expression and alternative splicing of biological clock-related genes in ovary during estrus and diestrus periods.In total,90 clock-related genes expressed in the ovaries of the Xiang pig were detected and 33 clock-related genes differentially expressed between estrous and diestrous stages.44 differential splicing events were identified from the transcripts of 34 biological clock-related genes.Furthermore,it was found 20 genes including the core clock components,arntl and cry1 were differentially regulated only at AS level and 14 genes,including per1 and clock,were differentially regulated at both expression and AS levels.We also proved that the core clock genes per1,cry1,clock and arntl and the clock-related genes,ppp1cb and ntrk1 were rhythmically expressed in Xiang pig ovaries by RT-qPCR experiments.The results demonstrated that the biological clock in the ovaries of Xiang pig might play an important role in regulating the ovarian physiological functions by the transcriptional and post-transcriptional regulation.关键词
昼夜节律/香猪/卵巢/发情周期/可变剪接Key words
circadian rhythm/Xiang pig/ovary/estrus cycle/alternative splicing分类
农业科技引用本文复制引用
唐靓婷,黄世会,牛熙,李升,王嘉福,冉雪琴..发情周期对香猪卵巢生物钟相关基因表达的影响[J].中国农业科技导报,2023,25(12):67-84,18.基金项目
National Natural Science Foundation of China(31960641,31672390) （31960641,31672390）
Guizhou Province Science and Technology Innovation Team Building Special(QKHPTRC[2019]5615) （QKHPTRC[2019]5615）
Guizhou Province"Hundred"Innovative Talents Project(2016-4012) （2016-4012）
Guizhou Agriculture Research program(QKHZC[2017]2585,QKHZC[2017]2587,QKHNY[2013]3073) （QKHZC[2017]2585,QKHZC[2017]2587,QKHNY[2013]3073）
National High Technology Research and Development Program of China(2013AA102503). （2013AA102503）
