中国药理学通报2023，Vol.39Issue(12)：2240-2246,7.DOI:10.12360/CPB202304066
CypD介导mPTP在褪黑素改善七氟醚重复暴露致认知损伤中的作用
Role of CypD-mediated mPTP in melatonin improving cognitive impairment induced by repeated exposure to sevoflurane
摘要
Abstract
Aim To investigate the involvement of cy-clophilin D(CypD)-mediated mitochondrial permea-bility transition pore(mPTP)in the neuroprotective effects of melatonin on cognitive impairment induced by repeated exposure to sevoflurane in newborn animals.Methods Mice were randomly assigned into control group,sevoflurane(Sevo)group,and melatonin pre-treatment+sevoflurane(Sevo+Mel)group.JC-1 kit was used to assess the mitochondrial membrane poten-tial(MMP);Western blot analysis was used to evalu-ate the protein expressions of CypD,postsynaptic den-sity protein 95(PSD95),and Synapsin-1;and behav-ioral test were employed to measure cognitive function.Results The MMP level in the Sevo group was signifi-cantly reduced compared to the control group(P＜0.01),the expression of CypD increased(P＜0.05),whereas the expression of PSD95 and Synapsin-1 de-creased(P＜0.01).Furthermore,the new object rec-ognition index and spatial memory ability both exhibi-ted a significant decline(P＜0.01,P＜0.05).How-ever,when compared to the Sevo group,Sevo+Mel group could raise the MMP level(P＜0.01),increase the expression of synaptic proteins(P＜0.05),de-crease the expression of CypD(P＜0.01)and elevate the new object recognition index and the spatial memo-ry capacity(P＜0.01).Conclusions Melatonin could ameliorate cognitive impairment induced by re-peated exposure to sevoflurane in newborn mice,and the underlying mechanism may be attributed to the in-hibition of mPTP mediated by CypD and the promotion of synaptic protein synthesis.关键词
褪黑素/七氟醚重复暴露/认知功能障碍/神经突触/亲环素D/线粒体通透性转化孔Key words
melatonin/repeated exposure to sevoflu-rane/cognitive impairment/neuronal synapse/cyclo-philin D/mitochondrial permeability transition pore分类
医药卫生引用本文复制引用
邹雪竹,张晓媛,张丽..CypD介导mPTP在褪黑素改善七氟醚重复暴露致认知损伤中的作用[J].中国药理学通报,2023,39(12):2240-2246,7.基金项目
安徽医科大学第二附属医院国家自然科学基金孵育计划项目(No 2020GQFY01) （No 2020GQFY01）
安徽省自然科学基金资助项目(No 1908085QH358) （No 1908085QH358）
