中国药理学通报2023，Vol.39Issue(12)：2274-2280,7.DOI:10.12360/CPB202305061
高糖环境中雄激素受体对心肌成纤维细胞增殖和脂质合成能力的影响
Effects of AR on proliferation and lipid synthesis of myocardial fibroblasts in high glucose environment
摘要
Abstract
Aim To explore the effect of androgen re-ceptor AR on the proliferation and lipid synthesis of cardiac fibroblasts under high-glucose conditions and the possible molecular mechanism.Methods The hearts of neonatal rats were dissected for primary cul-ture of cardiac fibroblasts.Then the growth status of CFs was observed under the inverted microscope,and the identification of CFs was performed by immunofluo-rescence staining using anti-vimentin.After cell adher-ence,the cells were divided into blank control group,high glucose model group,negative control group,and overexpressed AR group.The glucose concentration was 33.0 mmol·L-1except that the blank control group was 5.5 mmol·L-1.After 24 hours of CFs cul-ture,Western blot and RT-qPCR were used to detect the expression of AR,FASN,PCNA,cyclin D1,α-SMA,and collagen Ⅰ.Oil red O and CCK-8 were used to detect the changes in lipid synthesis and cell proliferation ability,respectively.Results Compared with the blank control group,the lipid synthesis and proliferation of CFs in the high glucose model group were enhanced.Western blot and RT-qPCR results showed that the expression of AR decreased,while the expression of fat lipid synthase(FASN),proliferation marker PCNA,cyclin D1 and fibrosis marker α-SMA and collagen Ⅰ increased.After AR overexpressed plasmid was transfected into the CFs treated by high glucose,AR overexpression markedly decreased the expression of FASN,PCNA,cyclin D1,α-SMA and collagen Ⅰ compared with the empty plasmid-trans-fected group.Meanwhile,oil red O staining and CCK-8 results showed that the lipid synthesis and prolifera-tion ability of the overexpressed AR group decreased compared with the empty vector group,respectively.Conclusions High glucose promotes the proliferation and lipid synthesis of cardiac fibroblasts.Besides,the mechanism may be related to the regulation of lipid synthesis regulated by AR.关键词
脂质合成/心肌纤维化/细胞增殖/心肌成纤维细胞/细胞代谢/雄激素受体Key words
lipid synthesis/myocardial fibrosis/cell proliferation/cardiac fibroblasts/cell metabolism/an-drogen receptor分类
医药卫生引用本文复制引用
温馨,周洋,林丽婵,刘芷言,宋凯,陶辉,臧洪梅..高糖环境中雄激素受体对心肌成纤维细胞增殖和脂质合成能力的影响[J].中国药理学通报,2023,39(12):2274-2280,7.基金项目
安徽省教育厅高校自然科学基金重大项目(No 2023AH040090) （No 2023AH040090）
安徽省转化医学研究基金项目(No 2021zhyx-C61) （No 2021zhyx-C61）
