肿瘤药学2023，Vol.13Issue(5)：574-581,8.DOI:10.3969/j.issn.2095-1264.2023.05.07
海莲叶提取物抑制非小细胞肺癌A549细胞增殖、侵袭、迁移并促进细胞凋亡
Extract of Bruguiera sexangula leaves inhibits the proliferation,invasion,migration and promotes the apoptosis of non-small cell lung cancer A549 cells
摘要
Abstract
Objective To study the effects of extract of Bruguiera sexangula leaves on the proliferation,apoptosis,inva-sion and migration of non-small cell lung cancer A549 cells.Methods Non-small cell lung cancer A549 cells were divided into Control group(treated with 0 μg·mL-1 extract of Bruguiera sexangula leaves),BSLE-L group(treated with 10 μg·mL-1 extract of Bruguiera sexangula leaves),BSLE-M group(treated with 20 μg·mL-1 extract of Bruguiera sexangula leaves),BSLE-H group(treated with 30 μg·mL-1 extract of Bruguiera sexangula leaves),BSLE-M+Jagged1-Fc group(treated with 20 μg·mL-1 extract of Bruguiera sexangula leaves and Notch signaling pathway activator Jagged1-Fc).MTT experiment was used to detect the proliferation of cells,and plate cloning experiment to detect the cell clone formation ability,flow cytometry to detect the cell apoptosis changes,Transwell chamber to detect the cell migration and invasion ability,Western blotting to detect the expressions of Bax,Bcl-2,vimentin,E-cadherin,MMP-2,Notch-1,NICD-1 and Hes-1 proteins.Results Com-pared with the Control group,the cell survival rate and the number of clone formation and the number of invasion and migra-tion cells decreased sequentially in the BSLE-L,BSLE-M and BSLE-H groups.The expression levels of Bax and E-cad-herin proteins in cells increased sequentially in the BSLE-L,BSLE-M and BSLE-H groups,and the expression levels of vi-mentin,MMP-2,Bcl-2,Notch-1,NICD-1 and Hes-1 proteins decreased(P<0.05).Compared with the BSLE-M group,the survival rate and the number of cell clone formation,and the number of migration and invasion cells increased significantly in the BSLE-M+Jagged1-Fc group,while the cell apoptosis rate decreased significantly.The expression levels of Bax and E-cadherin proteins in the cells were down-regulated,while the expression levels of Bcl-2,vimentin,MMP-2,Notch-1,NICD-1,Hes-1 proteins were up-regulated(P<0.05).Conclusion Bruguiera sexangula leaves extract inhibited the prolifer-ation,invasion,migration and EMT of non-small cell lung cancer A549 cells,and induced cell apoptosis.The mechanism was related to the decrease of the activation level of Notch signal.关键词
海莲叶提取物/非小细胞肺癌/迁移/凋亡/Notch信号通路Key words
Extract of Bruguiera sexangula leaves/Non-small cell lung cancer/Migration/Apoptosis/Notch signal pathway分类
医药卫生引用本文复制引用
李源,党海舟,刘慧..海莲叶提取物抑制非小细胞肺癌A549细胞增殖、侵袭、迁移并促进细胞凋亡[J].肿瘤药学,2023,13(5):574-581,8.基金项目
陕西省医学科学研究重点课题计划(2016JM1142). （2016JM1142）
