肿瘤药学2023，Vol.13Issue(5)：582-589,8.DOI:10.3969/j.issn.2095-1264.2023.05.08
川芎嗪对人结直肠癌SW480细胞增殖和凋亡的影响及机制研究
Effects and mechanism of tetramethylpyrazine on the proliferation and apoptosis of human colorectal cancer SW480 cell
摘要
Abstract
Objective To investigate the effects and mechanism of tetramethylpyrazine(TMP)on the proliferation and apoptosis of human colorectal cancer SW480 cells.Methods The human colorectal cancer SW480 cells in logarithmic growth phase were interfered with DMSO(blank control group),different concentrations of TMP(50,100,200 μg·mL-1)and L-OHP(25 μg·mL-1)for 48 h.Then the proliferation activity of SW480 cells in each group was detected by MTT,and the cloning ability of SW480 cells was observed by cell cloning experiment.The cell cycle,apoptosis and protein expression were detected by flow cytometry or Western blotting.Results Compared with blank control group,treatment with TMP(50,100,200 μg·mL-1)or L-OHP(25 μg·mL-1)could decrease the proliferation activity of SW480 cells,and increase the apop-tosis rate.Treatment with TMP(100,200 μg·mL-1)or L-OHP(25 μg·mL-1)could significantly inhibit the cell cloning abil-ity,block cell cycle in G0/G1 phase,increase the expression of PTEN,Cleaved Caspase-3,Bax,P27 and the ratio of Bax/Bcl-2,decrease the expression of p-Akt,cyclin D1,Bcl-2 and Akt phosphorylation rate,and all of the differences were statistically significant(P<0.05).Compared with L-OHP(25 μg·mL-1)treatment group,the proliferation activity of SW480 cells was decreased,the cell apoptosis rate increased,the expression of PTEN,Bax,P27 and the ratio of Bax/Bcl-2 increased,and the expression p-Akt,cyclin D1 and Akt phosphorylation rate decreased in the TMP 200 μg·mL-1 treatment group(P<0.05).Conclusion TMP may inhibit the PI3K/Akt pathway by upregulating the expression of PTEN,so that could in-hibit the proliferation and promote the apoptosis of SW480 cells.关键词
川芎嗪/结直肠癌/增殖/凋亡/PTEN/PI3K/Akt通路Key words
Tetramethylpyrazine/Colorecal cancer/Proliferation/Apoptosis/PTEN/PI3K/Akt pathway分类
医药卫生引用本文复制引用
王军,董魁,张文娟,李静,张向梅..川芎嗪对人结直肠癌SW480细胞增殖和凋亡的影响及机制研究[J].肿瘤药学,2023,13(5):582-589,8.基金项目
邯郸市科学技术研究与发展计划项目(1823208067ZC). （1823208067ZC）
