肿瘤药学2023，Vol.13Issue(5)：590-596,7.DOI:10.3969/j.issn.2095-1264.2023.05.09
山慈菇提取物对人结直肠癌SW480细胞迁移和侵袭能力的影响及其分子机制
The effects of extract of Shancigu on the migration and invasion of human colorectal cancer SW480 cell and its molecular mechanism
钟海川 1杨永强 1李可珍 2王彩丽 3彭亮4
作者信息
- 1. 万宁市人民医院 消化内镜科,海南 万宁,571500
- 2. 万宁市人民医院 影像科,海南 万宁,571500
- 3. 万宁市人民医院 消化内科,海南 万宁,571500
- 4. 万宁市人民医院 口腔科,海南 万宁,571500
- 折叠
摘要
Abstract
Objective To analyze the influence of the extract of Shancigu(appendiculate cremastra pseudobulb)on the migration and invasion ability of human colorectal cancer SW480 cell and its molecular mechanism.Methods The human colorectal cancer SW480 cell was selected and divided into blank control group,Shancigu groups of different concentra-tions(200 mg·L-1,400 mg·L-1),and 5-Fu group(10 μmol·L-1).The scratch experiment,cell proliferation inhibition ex-periment,cell migration and invasion experiment were performed to understand the migration and invasion ability of each group of human colorectal cancer SW480 cells.At the same time,the AEG-1 gene in SW480 cells was silenced by cell transfection technology,and the effect of AEG-1 gene silencing on the expression of various proteins in SW480 cells was understood by Western blotting detection.The expression changes of various proteins in SW480 cells treated with differ-ent concentrations of Shancigu extract was also observed.Results Compared with the blank control group,the wound heal-ing rate,cell migration rate and invasion rate of SW480 cells in different concentrations of Shancigu groups and 5-Fu group were significantly decreased,and the cell growth inhibition rate was significantly increased(P<0.05).The inhibition effect of Shancigu extract on the proliferation and invasion of rectal cancer cells was similar to that of 5-Fu.The inhibition effect on the migration and invasion rate of SW480 cells was concentration-dependent with Shancigu treatment,and the inhibition rate of cell proliferation was concentration-and time-dependent with Shancigu treatment.The expression levels of AEG-1,MMP-2 and MMP-9 proteins were significantly decreased in SW480 cells in the Shancigu groups and the 5-Fu group,and the expression level of E-cadherin protein was significantly increased(P<0.05).Conclusion The Shancigu extract may inhibit the migration and invasion of human colorectal cancer SW480 cells by inhibiting the expression of AEG-1 protein,up-regulating the expression of E-cadherin protein,and down-regulating the expression of MMP-2 and MMP-9 protein.关键词
结直肠癌/山慈菇/SW480细胞/迁移/侵袭/分子机制Key words
Colorectal cancer/Shancigu(appendiculate cremastra pseudobulb)/SW480 cell/Migration/Invasion/Mo-lecular mechanism分类
医药卫生引用本文复制引用
钟海川,杨永强,李可珍,王彩丽,彭亮..山慈菇提取物对人结直肠癌SW480细胞迁移和侵袭能力的影响及其分子机制[J].肿瘤药学,2023,13(5):590-596,7.
