中山大学学报（医学科学版）2023，Vol.44Issue(6)：958-964,7.
人牙龈间充质干细胞通过减少滤泡B细胞比例预防非肥胖糖尿病小鼠糖尿病
Human Gingival Stem Cells Prevent Diabetes in NOD Mice by Reducing Follicular B Cells
摘要
Abstract
[Background]Type 1 diabetes is caused by a chronic immune response that destroys islet beta cells,re-sulting in elevated blood glucose.Mesenchymal stem cells can prevent and treat the development of diabetes and its compli-cations.However,little is known about the effects and potential mechanisms of Gingival mesenchymal stem cells(GMSCs)in preventing diabetes.The aim of this study is to investigate the mechanism of GMSCs in preventing type 1 diabetes in mice and to find targets for clinical treatment of diabetes.[Methods]We injected human GMSCs into NOD mice to observe the trend of blood glucose,observed the survival of pancreatic β-cells by immunohistochemistry,and detected the change of immune cells in the spleen of mice by flow analysis.Finally,the immune cells in NOD mice were transfused into NOD-SCID mice to observe the onset of diabetes in NOD-SCID mice.[Results]GMSCs significantly reduced the incidence of di-abetes in NOD mice,with 64%of control mice developing diabetes at 27 weeks of age compared with 35%in the GMSC group,P=0.013.The percentage of Follicular B cells(FO B cell)in the spleen of GMSCs-treated mice decreased from(52.2±4.1)%to(43.2±5.3)%,P=0.008,while other types of immune cells did not change significantly.The immunohisto-chemical results showed that GMSCs could effectively improve the survival of pancreatic β-cells,which could continuous-ly produce insulin to control blood glucose.Finally,we found the spleen cells transfusion could prevent the development of diabetes in NOD-SCID mice.[Conclusion]GMSCs can reduce diabetes in mice by reducing FO B cells in the spleen.关键词
间充质干细胞/1型糖尿病/滤泡B细胞/NOD小鼠/血糖Key words
mesenchymal stem cell/type 1 diabetes/follicular B cell/NOD mice/blood glucose分类
医药卫生引用本文复制引用
郭义文,刘宝宁,余加,常鑫华,陈茂根,马毅,邓素雄,邓荣海..人牙龈间充质干细胞通过减少滤泡B细胞比例预防非肥胖糖尿病小鼠糖尿病[J].中山大学学报（医学科学版）,2023,44(6):958-964,7.基金项目
国家自然科学基金(81401324,81770410),广东省自然科学基金(2018A030313611),广东省器官捐献和移植免疫重点实验室(2013A061401007,2017B030314018,2020B1212060026) （81401324,81770410）
