miR-122-5p靶向调控人骨髓间充质干细胞诱导成骨分化的分子机制研究OA

Objective:To explore the role and mechanism of miR-122-5p on the osteogenic differentiation of hBMSCs.Methods:we investigated the role of miR-122-5p in osteogenic differentiation by alkaline phosphatase staining and Alizarin red staining,Dual luciferase reporter gene assay was used to verify the targeting relationship between miR-122-5p and BRCC3,Expression of Runx2、Osterix、wnt3a、β-catenin、GSK3-β、miR-122-5 and BRCC3 mRNA in hBMSCs were detected by quantitative reverse transcription polymerase chain reaction(RT-qPCR).Western blot was used to de-tect the expression of β-catenin protein.Results:The over expression of MiR-122-5p significantly promoted the activation of alkaline phosphatase(ALP)and formation of calcified nodules in osteoblasts,The mRNA expressions of β-catenin、Runx2、Osterix、wnt3a、GSK3-β and the protein expres-sion of β-catenin were significantly higher than those in control group.The dual-luciferase reporter gene experiment confirmed that BRCC3 was the downstream target gene of miR-122-5p.At the same time,inhibited osteogenic differentiation was also observed after BACC3 overexpression,the ex-pression levels ofβ-catenin、Runx2、Osterix、wnt3a、GSK3-β were inhibited.Conclusion:miR-122-5p can promote osteogenic differentiation of hBM-SCs,and targeting BACC3affects the activation of the wnt/β-catenin signaling pathway and regulates the process of osteoporosis treatment.