中医康复2024，Vol.1Issue(1)：26-31,6.DOI:10.19787/j.issn.2097-3128.2024.01.006
miR-122-5p靶向调控人骨髓间充质干细胞诱导成骨分化的分子机制研究
Study on The Molecular Mechanism of MiR-122-5p Targeted Regulation of Osteogenic Differentiation Induced by Human Bone Marrow Mesenchymal Stem Cells
摘要
Abstract
Objective:To explore the role and mechanism of miR-122-5p on the osteogenic differentiation of hBMSCs.Methods:we investigated the role of miR-122-5p in osteogenic differentiation by alkaline phosphatase staining and Alizarin red staining,Dual luciferase reporter gene assay was used to verify the targeting relationship between miR-122-5p and BRCC3,Expression of Runx2、Osterix、wnt3a、β-catenin、GSK3-β、miR-122-5 and BRCC3 mRNA in hBMSCs were detected by quantitative reverse transcription polymerase chain reaction(RT-qPCR).Western blot was used to de-tect the expression of β-catenin protein.Results:The over expression of MiR-122-5p significantly promoted the activation of alkaline phosphatase(ALP)and formation of calcified nodules in osteoblasts,The mRNA expressions of β-catenin、Runx2、Osterix、wnt3a、GSK3-β and the protein expres-sion of β-catenin were significantly higher than those in control group.The dual-luciferase reporter gene experiment confirmed that BRCC3 was the downstream target gene of miR-122-5p.At the same time,inhibited osteogenic differentiation was also observed after BACC3 overexpression,the ex-pression levels ofβ-catenin、Runx2、Osterix、wnt3a、GSK3-β were inhibited.Conclusion:miR-122-5p can promote osteogenic differentiation of hBM-SCs,and targeting BACC3affects the activation of the wnt/β-catenin signaling pathway and regulates the process of osteoporosis treatment.关键词
miR-122-5p/人骨髓间充质干细胞/成骨分化/wnt/β-catenin信号通路Key words
MiR-122-5p/BRCC3/wnt/β-catenin signaling/osteoporosis分类
医药卫生引用本文复制引用
蔡立雄,缪忠绿..miR-122-5p靶向调控人骨髓间充质干细胞诱导成骨分化的分子机制研究[J].中医康复,2024,1(1):26-31,6.基金项目
佛山市科技局医学类科技攻关项目(NO.1920001001298) （NO.1920001001298）
