活血消痛酒质量标准及制备工艺研究OA

Objective:To establish quality standard and optimize the preparation technology of Huoxue Xiaotong liquor.Methods:Thin layer chromatography(TLC)was used to identify sovereign drug including Zanthoxylum nitidum(Roxb.)DC and Panax notoginseng and identify minister drug:Resina Draconis;High Performance Liquid Chromatography(HPLC)was used to determine the content of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1.Chromatographic column is Waters X Bridge Shield RPC18(4.6×250 mm,5 μm)colum.Mobile phase consisting of water(A)-acetonitrile(B)and gradient elution(0～15 min,20%～23%B;15～25 min,23%B;25～35 min,23%～40%B;35～36 min,40%～90%B).The flow rate was 1 mL/min,column temperature was 30℃,detection wavelength was 203 nm,the injection volume was 10 μL;Central composite design(CCD)-response surface method was used to optimize the preparation technology of Huoxue Xiaotong liquor.Results:The characteristic spots of Zanthoxylum nitidum(Roxb.)DC、Resina Draconis and Panax notoginseng were clear and well separated,and there was no interference from the negative control.The notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 had a good linear relationship within their respective concentration ranges(r2>0.999).There was no interference from the negative control,and the results of precision,stability,repeatability and recovery rate were good.The total content of three saponins in 10 batches of samples ranged from 0.4225～0.5982 mg/mL;The CCD method was used along with actual situation to obtain the optimal process as follows:alcohol content was 42%,material ratio was 16.7 mg/mL,soaking time was 7 days.Conclusion:The method established in this study is simple,accurate and reliable,and can be used for the study of quality standard of Huoxue Xiaotong liquor.At the same time,the optimal technology of Huoxue Xiaotong liquor was found scientifically,comprehensively and accurately by the method of central composite design.