北华大学学报（自然科学版）2024，Vol.25Issue(1)：35-39,5.DOI:10.11713/j.issn.1009-4822.2024.01.006
桦褐孔菌品质鉴定及抗氧化活性研究
Quality Identification and Antioxidant Activity of Inonotus obliquus
摘要
Abstract
Objective To determine the best extraction technology of Inonotus obliquus polysaccharides and evaluate its antioxidant activity.Method The polysaccharides of Inonotus obliquus were extracted by water extraction,ultrasonic method,microwave method and enzymatic method for three times,and the reduction ability of Inonotus obliquus polysaccharides to FRAP,DPPH·,·OH and ABTS·was determined compared with VC.Results When the ratio of material to liquid was 1 ∶ 20 g/mL,ultrasonic power 400 W,extraction temperature 80℃and extraction time 90 min,the highest extraction rate of polysaccharide was the highest,reached 10.35%.The results of antioxidant activity study showed that when the concentration of polysaccharide of Inonotus obliquus was 400 μg/mL,its total antioxidant capacity was equivalent to 244.04 μmol/L FeSO4;The scavenging rate of DPPH·was weak,and the scavenging rate of·OH and ABTS·was more than 80%.Conclusion Ultrasonic extraction is the best among the four methods,and the antioxidant activity of Inonotus obliquus polysaccharides is higher.关键词
桦褐孔菌/多糖/抗氧化Key words
Inonotus obliquus/polysaccharides/antioxidant分类
医药卫生引用本文复制引用
常明珠,李亭昱,崔云鹤,刘腾达,郭笑,母润红..桦褐孔菌品质鉴定及抗氧化活性研究[J].北华大学学报（自然科学版）,2024,25(1):35-39,5.基金项目
吉林省科技发展计划项目(20210204185YY,YDZJ202201ZYTS287) （20210204185YY,YDZJ202201ZYTS287）
北华大学研究生创新项目(2022017). （2022017）
