北华大学学报（自然科学版）2024，Vol.25Issue(1)：40-43,4.DOI:10.11713/j.issn.1009-4822.2024.01.007
丙戊酸钠促进神经根回植术后神经元存活的机制研究
Neuroprotection Role of Sodium Valproate after Nerve Root Re-implantation
摘要
Abstract
Objective To explore the protective effect of Sodium valproate on spinal cord neurons in rats with brachial plexus root avulsion after nerve root re-implantation.Method Adult rats were randomly divided into three groups:blank group,operation group and valproate group.The operation group and the valproate group were treated with nerve root replantation after brachial plexus root avulsion injury,and the rats in valproate group was fed with Sodium volproate by drinking water after operation.The corresponding segments of spinal cord were collected at 1 d,2 d,3 d,7 d and 14 d after operation,then apoptotic of neurons was detected by TUNEL and the expression of Bcl-2 was detected by Western blot and real-time quantitative PCR.Results No apoptotic cells were found at 1 d and 2 d after surgery by TUNEL detection,and apoptotic neurons were found at 3 d to 14 d after surgery,the percentage of apoptotic neurons in the valproate group was less than that in the operation group at 3 d and 7 d after operation(P＜0.05).It was found by Western blot that Bcl-2 protein expression in the valproate group was higher than that in the operation group at 2 d,3 d,7 d after operation(P＜0.05).By Real-time PCR detection found Bcl-2 mRNA expression in valproate group is higher than that in operation group at 2 d,3 d,7 d after operation(P＜0.05).Conclusion The early application of Sodium valproate can inhibit the apoptosis of neurons after the re-implantation of nerve roots in brachial plexus avulsion injury,and the mechanism may be achieved by promoting the expression of Bcl-2 in the spinal cord and inhibiting the apoptosis signal transduction pathway.关键词
丙戊酸钠/根性撕脱伤/神经根回植/Bcl-2Key words
Sodium valproate/root avulsion/nerve root re-implantation/Bcl-2分类
医药卫生引用本文复制引用
吴殿秀,袁依然,杨丽娟,王海鹏..丙戊酸钠促进神经根回植术后神经元存活的机制研究[J].北华大学学报（自然科学版）,2024,25(1):40-43,4.基金项目
吉林省教育厅科学技术研究项目(JJKH20230080KJ,JJKH20220068KJ) （JJKH20230080KJ,JJKH20220068KJ）
吉林省卫生与健康青年骨干培养计划项目(2020Q004) （2020Q004）
吉林省大学生创新创业项目(S202210201157). （S202210201157）
