北华大学学报（自然科学版）2024，Vol.25Issue(1)：44-47,4.DOI:10.11713/j.issn.1009-4822.2024.01.008
miRNA-758-3p对口腔鳞状细胞癌细胞增殖、迁移和侵袭的影响
Effect of miRNA-758-3p on Proliferation,Migration and Invasion of Oral Squamous Cell Carcinoma
摘要
Abstract
Objective Explore the expression of microRNA-758-3p in oral squamous cell carcinoma and its impact on proliferation,migration and invasion.Method Real time quantitative PCR(qRT-PCR)was used to detect the expression level of miR-758-3p in normal gingival epithelial cells(HGE)and oral squamous cell carcinoma cell lines(CAL27,SCC9 and SCC25).Transfect miR-758-3p mimetics into CAL27 cells to construct an overexpression miR-758-3p cell model,and use WST-1 to detect cell proliferation ability;Transwell method for detecting the impact of migration and invasion;The effect of overexpression of miR-758-3p on MMP2 and MMP9 protein levels in CAL27 cells was detected by Western blot.Results Compared with HGE cells,the expression of miR-758-3p was downregulated in oral squamous cell carcinoma cell lines CAL27,SCC9 and SCC15(P＜0.05),with the lowest expression of miR-758-3p in CAL27 cells.After transfection with miR-758-3p mimetics,the expression level of miR-758-3p in CAL27 cells significantly increased.Overexpression of miR-758-3p significantly inhibited the cell proliferation,migration and invasion ability of CAL27(P＜0.05);significantly inhibited the expression of MMP2 and MMP9 proteins(P＜0.05).Conclusion The expression of miR-758-3p is relatively low in oral squamous cell carcinoma cell line CAL27,and overexpression of miR-758-3p can significantly inhibit the proliferation,migration and invasion of CAL27 cells.关键词
miR-758-3p/增殖/迁移/侵袭/口腔鳞状细胞癌Key words
miR-758-3p/proliferation/migration/invasion/oral squamous cell carcinoma分类
医药卫生引用本文复制引用
张越,杨超..miRNA-758-3p对口腔鳞状细胞癌细胞增殖、迁移和侵袭的影响[J].北华大学学报（自然科学版）,2024,25(1):44-47,4.基金项目
吉林省卫生健康科技能力提升项目(2022LC064) （2022LC064）
吉林省中医药科技项目(2023066). （2023066）
