北华大学学报（自然科学版）2024，Vol.25Issue(1)：60-65,6.DOI:10.11713/j.issn.1009-4822.2024.01.011
GSTP1和ERCC1基因多态性与食管癌患者放疗敏感性及预后的关系
Relationship between GSTP1 and ERCC1 Gene Polymorphisms and Radiation Sensitivity and Prognosis in Esophageal Cancer Patients
摘要
Abstract
Objective To explore the relationship between glutathione S-transferase P1 gene(GSTP1)and excision repair cross-complementation group 1 gene(ERCC1)polymorphisms and the radiosensitivity and prognosis of patients with esophageal cancer.Method A total of 200 patients with esophageal cancer who received docetaxel monotherapy and concurrent radiotherapy were selected.After one course of radiotherapy,they were divided into radiotherapy sensitive group(complete remission,partial remission)and radiotherapy non-sensitive group(stable,progression)according to complete remission,partial remission,stable and progression.Follow-up was conducted for 3 years to record survival of patient.Peripheral venous blood samples were collected,genomic DNA was extracted and PCR amplification was performed to analyze the single nucleotide polymorphisms(SNP)of GSTP1 gene G28152A site and ERCC1 gene C118T site.Adequate amounts of esophageal cancer tissues were obtained,and after tissue homogenization,the concentrations of myelin basic protein 1(MBP-1),phosphatase and tensin homolog(PTEN),G1/S-specific Cyclin D1,and phospholipase Cε1(PLCE1)gene proteins were measured by using enzyme-linked immunosorbent assay.Results The proportion of patients with esophageal cancer carrying heterozygous GA and mutant AA genotypes of GSTP1 gene who achieved complete remission or partial remission after radiotherapy was significantly higher than that of patients with wild-type GG genotype,and the proportion of patients with stable or progression was significantly lower than that of patients with wild-type GG genotype(P＜0.01).The proportion of patients with esophageal cancer carrying wild-type CC genotype of ERCC1 gene who achieved complete remission or partial remission after radiotherapy was significantly higher than that of patients carrying heterozygous CT and mutant TT genotypes(P＜0.01).The survival rate of patients with esophageal cancer carrying heterozygous GA and mutant AA genotypes of GSTP1 gene after radiotherapy increased(P＜0.05);The survival rate of patients with esophageal cancer carrying wild-type CC genotype of ERCC1 gene after radiotherapy significantly increased(P＜0.05).The expression levels of apoptosis-related genes MBP-1 and PTEN significantly decreased,while the expression levels of proliferation-related genes Cyclin D1 and PLCE1 significantly increased in the radiotherapy non-sensitive group(P＜0.05).The expression levels of MBP-1 and PTEN significantly increased,and the expression levels of Cyclin D1 and PLCE1 significantly decreased in patients with esophageal cancer carrying heterozygous GA and mutant AA genotypes of GSTP1 gene(P＜0.01).The expression levels of MBP-1 and PTEN significantly increased,and the expression levels of Cyclin D1 and PLCE1 significantly decreased in patients with esophageal cancer carrying wild-type CC genotype of ERCC1 gene(P＜0.05).Conclusion Patients with esophageal cancer carrying heterozygous GA and mutant AA genotypes of GSTP1 gene and wild-type CC genotype of ERCC1 gene have better prognosis after radiotherapy,which may be related to upregulation of apoptosis-related genes MBP-1 and PTEN expression and downregulation of proliferation-related genes Cyclin D1 and PLCE1 expression.关键词
食管癌/GSTP1 基因/ERCC1 基因/放疗/敏感性/凋亡相关基因/增殖相关基因/预后Key words
esophageal cancer/GSTP1 gene/ERCC1 gene/radiotherapy/sensitivity/apoptosis related genes/proliferation related genes/prognosis分类
医药卫生引用本文复制引用
李强虎,辛勇..GSTP1和ERCC1基因多态性与食管癌患者放疗敏感性及预后的关系[J].北华大学学报（自然科学版）,2024,25(1):60-65,6.基金项目
江苏省级学科带头人项目(DTRB202011). （DTRB202011）
