影像科学与光化学2023，Vol.41Issue(6)：350-357,8.DOI:10.7517/issn.1674-0475.230808
近红外荧光成像监测EN01 mAb在宫颈癌荷瘤小鼠体内生物分布的研究
Study on the Biological Distribution of ENO1 mAb in Cervical Cancer Bearing Mice Monitored by Near Infrared Fluorescence Imaging
摘要
Abstract
In this study,the distribution,metabolism and tumor targeting efficiency of Enolase 1(ENO1)monoclonal antibody(mAb)in cervical cancer bearing mice were investigated by near infrared fluorescence imaging.The relationship between ENO1 expression and tumor stage,prognosis and tumor immune invasion was analyzed by cancer genome atlas.Cy7-NHS and FITC were coupled with ENOl mAb to form Cy7-ENO1 mAb and FITC-ENOl mAb,and the localization of ENOl mAb in cervical cancer cell TC-1 was verified by laser confocal microscopy,and the subcutaneous tumor model of cervical cancer was established.Near infrared fluorescence(NIRF)imaging was performed at 1,6,24,48,72.and 120 h after caudal intravenous injection of Cy7-ENO1 mAb.After 24 h of imaging,major organs were rapidly dissected for in vitro imaging.Bioinformatics results showed significant differences in the expression of ENOl between normal cervical tissue and cervical cancer tissue while immunohistochemistry(IHC)indicated higher ENOl protein expression in cervical cancer tissues.In addition,the expression of ENOl was significantly correlated with tumor stage and lymph node stage,and the high expression of ENO1 was correlated with multiple glycolytic enzymes.Confocal laser microscopy showed that FITC-ENOl mAb could specifically bind to the surface of TC-1 cell membrane,and no cell internalization was observed.Near infrared fluorescence imaging showed that Cy7-ENO1 mAb was mainly enriched in liver,kidney and tumor tissues,and had high tumor targeting efficiency.NIRF imaging allows real-time and dynamic monitoring of ENOl mAb distribution in cervical cancer bearing mice,whose metabolic organs are mainly liver and kidney,and confirms ENOl mAb's targeting of cervical cancer.关键词
近红外荧光成像/ENO1 mAb/生物分布/Cy7-NHSKey words
near infrared fluorescence imaging/ENOl mAb/biodistribution/Cy7-NHS引用本文复制引用
赵调红,章婷婷,陈圆圆,路如霞,赵源旭,裴亚萍,刘会玲,祝秉东..近红外荧光成像监测EN01 mAb在宫颈癌荷瘤小鼠体内生物分布的研究[J].影像科学与光化学,2023,41(6):350-357,8.基金项目
国家自然科学基金地区基金(82260557),北京科创医学发展基金会肿瘤研究基金面上项目(KC2021-JX-0186-141) （82260557）
