广东药科大学学报2023，Vol.39Issue(6)：24-36,13.DOI:10.16809/j.cnki.2096-3653.2023100702
基于网络药理学-分子对接及实验研究探讨高良姜-香附药对治疗原发性痛经的作用机制
Mechanism of Alpinia officinarum-Cyperus rotundus in the treatment of primary dysmenorrhea based on network pharmacology,molecular docking and experimental study
摘要
Abstract
Objective To explore the mechanism of Alpinia officinarum-Cyperus rotundus(Referred to as"Liangfu")in the treatment of primary dysmenorrhea(PD)based on network pharmacology,molecular docking and experimental study.Methods The active components of Alpinia officinarum and Cyperus rotundus were obtained by TCSSMP and literature search,and the corresponding targets were obtained from TCSMP and the Swiss Target Prediction platform."Primary dysmenorrhea"and"dysmenorrhea"were used as the keywords to search the corresponding targets in the GeneCard,DrugBank,DisGenet,TTD,and OMIM databases.STRING database was used to build a PPI network.Enrichment analysis of KEGG and GO was carried out by Metascape platform.The"component-target-pathway"network was constructed by taking Alpinia officinarum and Cyperus rotundus intersection target,corresponding active components,and related pathways.Then the main compounds and Core target in the"component-target-pathway"and PPI networks were used for docking.PD mouse model was established by treatment with combining estradiol benzoate and oxytocin,and the writhing reaction was observed.HE staining was used to observe the pathological changes in the mouse uterus.The content of prostaglandin E2(PGE2),prostaglandin F2α(PGF2α),and β-endorphin(β-EP)in serum was detected by ELISA.The expression of protein and mRNA was verified by western blot and RT-qPCR.Results Liangfu contained 29 active ingredients,and the key targets included STAT3,PIK3CA,AKT1,ESR1,TP53,and PTGS2.The molecular docking showed that the key components and core targets had strong binding capacity.The results of animal experiments showed that the Liangfu group alleviated the edema,congestion,and inflammation of uterine tissues in PD mice,reduced the level of PGF2αin serum,increased the level of PGE2 and β-EP,and inhibited the expression of COX-2,p38,PI3K,and p-AKT mRNA and protein.Conclusion Liangfu can relieve the pain caused by PD,which may be related to modulation of PI3K/AKT,MAPK,and PTGS2 pathways.关键词
高良姜/香附/原发性痛经/网络药理学/分子对接/作用机制Key words
Alpinia officinarum/Cyperus rotundus/primary dysmenorrhea/network pharmacology/molecular docking/mechanism of action分类
医药卫生引用本文复制引用
黄玉芳,谭银丰,任喜康,李永辉,张俊清,李海龙..基于网络药理学-分子对接及实验研究探讨高良姜-香附药对治疗原发性痛经的作用机制[J].广东药科大学学报,2023,39(6):24-36,13.基金项目
海口市重点科技项目(2020-048) （2020-048）
