海南医学院学报2023，Vol.29Issue(23)：1761-1766,6.DOI:10.13210/j.cnki.jhmu.20231008.001
蒺藜皂苷对A549细胞增殖及侵袭能力影响的研究
Effects of Tribulus terrestris saponins on proliferation and invasion of A549 cells
摘要
Abstract
Objective:To investigate the effects of Tribulus terrestris saponin on the proliferation and invasion ability of non-small cell lung cancer A549 cells.Methods:A549 cells were divided into normal control and experimental groups(Tribulus terrestris saponin 250 μg/mL group,Tribulus terrestris saponin 200 μg/mL group,Tribulus terrestris saponin 150 μg/mL group,Tribulus terrestris saponin 100 μg/mL group,Tribulus terrestris saponin 50 μg/mL group).The proliferation viability of the cells in each group was detected by CCK8,the invasion of tumor cells was detected by Transwell analysis.The mRNA expression of MMP9 and caspase-3 in each group of cells was detected by RT-PCR.Immunofluorescence staining was used to observe the fluo-rescence intensity of caspase-3 in each group of cells.Results:Compared with the normal control group,Tribulus terrestris sapo-nin significantly inhibited the proliferation activity and invasion ability of A549 cells,which was statistically significant(P<0.01).In the invasion assay,compared with the control group,MMP9 expression was significantly reduced and caspase-3 expression was significantly increased in the Tribulus terrestris saponin group,in a concentration-dependent manner,with statistically significant differences(P<0.01).By cellular immunofluorescence staining experiments,it was found that the fluorescence expression of cas-pase-3 was enhanced in the experimental group compared with the normal control group,in which the high concentration saponin group was significantly higher than the low concentration group.Conclusion:Tribulus terrestris saponin can inhibit the invasive ability of A549 cells by downregulating the expression of MMP9,and induce irreversible apoptosis by upregulating the activation of caspase-3 expression.关键词
蒺藜皂苷/非小细胞癌/含半胱氨酸的天冬氨酸蛋白水解酶/基质金属蛋白酶Key words
Tribulus terrestris saponin/Non-small cell carcinoma/Cysteinyl aspartate specific proteinase/Matrix metallo-proteinase分类
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唐欢欢,黄亚楠,卢在庆,周向东,张海滨,李琪,刘合露..蒺藜皂苷对A549细胞增殖及侵袭能力影响的研究[J].海南医学院学报,2023,29(23):1761-1766,6.基金项目
This study was supported by National Key R&D Plan(2022YFC2305004) （2022YFC2305004）
Hainan Province Major Science and Technology Special Project(ZDKJ2021036) （ZDKJ2021036）
Key R&D projects in Hainan Province(ZDYF2020223) （ZDYF2020223）
Hainan Province Key R&D Plan International Science and Technology Cooperation Project(GHYF2022011) （GHYF2022011）
Hainan Provincial Innovation Team Project(820CXTD448) （820CXTD448）
National Natural Science Foundation of China(82260001,82160012) 国家重点研发计划(2022YFC2305004) （82260001,82160012）
海南省重大科技专项(ZDKJ2021036) （ZDKJ2021036）
海南省重点研发项目(ZDYF2020223) （ZDYF2020223）
海南省重点研发计划国际科技合作项目(GHYF2022011) （GHYF2022011）
海南省创新团队项目(820CXTD448) （820CXTD448）
国家自然科学基金资助项目(82260001,82160012) （82260001,82160012）
