海南医学院学报2023，Vol.29Issue(23)：1767-1773,7.DOI:10.13210/j.cnki.jhmu.20231023.003
PRPF18对肝癌的影响及药敏分析
Effect and drug sensitivity analysis of PRPF18 on HCC
摘要
Abstract
Objective:To analyze the effects of PRPF18 on the proliferation,migration,invasion and apoptosis of hepato-cellular carcinoma cell line MHCC97H and drug sensitivity analysis.Methods:The expression,prognosis and drug sensitivity of PRPF18 in hepatocellular carcinoma have been analyzed by using bioinformatics.The expression of PRPF18 in HCC and normal hepatocytes was verified by qRT-PCR.PRPF18 was silenced by transient transfection in MHCC97H cells,and the silencing ef-fect was verified by qRT-PCR.CCK-8 assay and colony formation assay were used to analyze the effect of silencing PRPF18 on the proliferation of MHCC97H cells.The effects of silencing PRPF18 on the migration and invasion of MHCC97H cells were ana-lyzed by Transwell assay and scratch assay.Hoechst 33342 staining and mitochondrial membrane potential assay were used to ana-lyze the effect of silencing PRPF18 on apoptosis of MHCC97H cells.Results:PRPF18 was highly expressed in HCC and signifi-cantly associated with the prognosis of patients.Patients with high expression of PRPF18 had higher sensitivity to tipifarnib and sunitinib.After silencing PRPF18,the proliferation,migration and invasion of MHCC97H cells were significantly lower than those of the control group.Silencing PRPF18 reduced mitochondrial membrane potential and promoted apoptosis.Conclusion:PRPF18 has a good prognostic value in HCC.Silencing PRPF18 may inhibit the proliferation,migration and invasion of MHCC97H cells and promote apoptosis.关键词
肝癌/PRPF18/增殖/迁移/侵袭/凋亡Key words
Hepatocellular carcinoma/PRPF18/Proliferation/Migration/Invasion/Apoptosis分类
医药卫生引用本文复制引用
张朝升,徐子云,范玉纯,农敏玉,黄佳怡,蒋利和..PRPF18对肝癌的影响及药敏分析[J].海南医学院学报,2023,29(23):1767-1773,7.基金项目
This study was supported by the Funding for the High-Level Talent Introduction Project of Youjiang Ethnic Medical College(yy2021sk002) （yy2021sk002）
Key R&D Project in Qingxiu District,Nanning City(2020023) （2020023）
2021 Guangxi Zhuang Autonomous Region Regional College Student Innovation and Entrepreneurship Training Program Project(S202110599073) 右江民族医学院高层次引进人才项目经费(yy2021sk002) （S202110599073）
南宁市青秀区重点研发项目(2020023) （2020023）
2021年广西壮族自治区区级大学生创新创业训练计划项目(S202110599073) （S202110599073）
