海南医学院学报2023，Vol.29Issue(23)：1774-1779,6.DOI:10.13210/j.cnki.jhmu.20230825.005
复方扶芳藤合剂影响免疫抑制小鼠CD11b+单核细胞CD69的表达
Compound fufangteng mixture affects the expression of CD69 on CD11b + monocytes in immunosuppressed mice
摘要
Abstract
Objective:To investigate the effects of compound fufangteng mixture(CFM)on the expression levels of CD11b+ monocytes surface activation molecules CD2,CD69 and depleted molecules PD-1 and CD95 in spleen and peripheral blood of immunosuppressed mice.Methods:A total of 30 healthy SPF Balb/c male mice were randomly divided into the control group,the immunosuppressive model group(CTX group),the high,medium and low dose intervention group(CTX+CFM-H group,CTX+CFM-M group,CTX+CFM-L group),the Astragalus particles(AP)intervention group(CTX+AP group).The mice in each intervention group were injected intraperitoneally with cyclophosphamide(CTX)70 mg/kg for 3 consecutive days,and then administered for 10 days according to the group regimen,and the materials were taken 24 hours after the last dose.Flow cytometry detected the expression of CD11b+ monocytes surface activation molecules(CD2,CD69)and depleted molecules(PD-1,CD95)in mouse peripheral blood.Results:Compared with the control group,the expression levels of CD11b+ and CD69+ in the spleen of CTX+CFM-H,CTX+CFM-M and CTX+CFM-L groups showed an improvement trend,and de-creased with the dose gradient of CFM.Conclusion:CFM could regulate the immune function of splenic CD11b+ CD69 cells in immunosuppressed mice.关键词
复方扶芳藤合剂/免疫抑制/CD69/CD95Key words
Compound fufangteng mixture/Immunosuppression/CD69/CD95分类
医药卫生引用本文复制引用
丁健,陈果,李建明,李开奇,杨晨一,伍月榕..复方扶芳藤合剂影响免疫抑制小鼠CD11b+单核细胞CD69的表达[J].海南医学院学报,2023,29(23):1774-1779,6.基金项目
This study was supported by National Natural Science Foundation Project(81860780) （81860780）
Guangxi Natural Science Foundation General Project(2020GXNSFAA297162) 国家自然科学基金项目(81860780) （2020GXNSFAA297162）
广西自然科学基金面上项目(2020GXNSFAA297162) （2020GXNSFAA297162）
