海南医学院学报2023，Vol.29Issue(23)：1809-1816,8.DOI:10.13210/j.cnki.jhmu.20230901.004
基于生物信息学分析SPHK1在胃癌中的表达及临床价值
SPHK1 expression in gastric cancer and clinical value based on bioinformatics analysis
摘要
Abstract
Objective:To elucidate the expression of SPHK1 in gastric cancer tissues and to analyse its clinical value in gas-tric cancer using bioinformatics.Methods:The expression of SPHK1 in gastric cancer tissues was analysed using HPA and TCGA databases.The association between the expression of both SPHK1 and MKI67 in gastric cancer was analysed using GEPIA data-base.Relationship between SPHK1 mRNA expression level and survival of gastric cancer patients was analysed based on Kaplan-Meier Plotter database.Gene enrichment pathway predicted the potential biological function of SPHK1 on gastric cancer.The TIMER database was used to predict the expression of SPHK1 in immune-infiltrating cells.The expression of SPHK1 in gastric cancer tissues was analysed by IHC and Western Blot.Results:Bioinformatics analysis showed that SPHK1 was highly expressed in gastric cancer tissues compared to paracancerous tissues(P<0.001).The analysis from the GEPIA database reveals a signifi-cant increase in the expression of SPHK1and MKI67 in gastric cancer tissues(P<0.05).Furthermore,there is a positive correla-tion between the two proteins(P=0.00 049).Kaplan-Meier Plotter survival analysis showed that high expression of SPHK1 pre-dicted worse overall survival tine(logrank P=0.002)and post-progression generation time(logrank P=0.0019)in gastric cancer.Gene enrichment pathway results showed that SPHK1 may be involved in multiple gastric cancer progression pathways.TIMER database analysis showed that in macrophages,gastric cancer patients with low SPHK1 expression group had a better post-progression period than those with high SPHK1 expression group(P=0.002).IHC showed that the staining intensity and staining positive area of SPHK1 in gastric cancer tissues were higher than that of adjacent non-tumour tissues.And the staining in-tensity of TNMI and Ⅱ stages was weaker than that of Ⅲ and Ⅳ stages.Western blot analysis revealed higher levels of SPHK1 protein in gastric cancer tissue compared to paired adjacent non-cancerous tissue in 9 cases(P=0.0 265).Conclusion:SPHK1 gene is highly expressed in gastric cancer tissues and is associated with poor prognosis and may reduce tumour immunosuppres-sion,which may serve as a potential marker for evaluating the prognosis of gastric cancer patients.关键词
胃癌/SPHK1/生物信息学/预后Key words
Stomach adenocarcinoma/SPHK1/Pioinformatics/Prognosis分类
医药卫生引用本文复制引用
凌潜龙,管佳佳,纪凯,朱爽秋,朱冰..基于生物信息学分析SPHK1在胃癌中的表达及临床价值[J].海南医学院学报,2023,29(23):1809-1816,8.基金项目
This study was supported by Anhui Province Higher Education Natural Science Research Project(KJ2021ZD0090)and Bengbu Medical College Graduate Research Innovation Project(Byycx22090) 安徽省高等学校自然科学研究项目(KJ2021ZD0090) （KJ2021ZD0090）
蚌埠医学院研究生科研创新项目(Byycx22090) （Byycx22090）
