首页|期刊导航|郑州大学学报（医学版）|Transgelin-2对牙龈卟啉单胞菌感染的食管鳞状细胞癌细胞恶性生物学行为的影响

Transgelin-2对牙龈卟啉单胞菌感染的食管鳞状细胞癌细胞恶性生物学行为的影响

伍当柔高社干齐义军朱巧晴朱亚飞许海军陈攀王明赵云岗孙丹妮郭苒

郑州大学学报（医学版）2023，Vol.58Issue(6)：741-745,5.

郑州大学学报（医学版）2023，Vol.58Issue(6)：741-745,5.DOI:10.13705/j.issn.1671-6825.2022.12.070

Transgelin-2对牙龈卟啉单胞菌感染的食管鳞状细胞癌细胞恶性生物学行为的影响

Effects of Transgelin-2 silencing on malignant biological behavior of e-sophageal squamous cell carcinoma cells infected by Porphyromonas gin-givalis

伍当柔 ¹高社干 ¹齐义军 ¹朱巧晴 ¹朱亚飞 ²许海军 ¹陈攀 ¹王明 ³赵云岗 ⁴孙丹妮 ⁵郭苒¹

作者信息

1. 河南科技大学临床医学院||河南科技大学第一附属医院||河南省肿瘤表观遗传重点实验室||河南省微生态与食管癌防治重点实验室河南洛阳 471003
2. 北京垂杨柳医院老年医学科北京 100022
3. 郑州大学第一附属医院医学研究中心郑州 450052
4. 长治市妇幼保健院医学遗传科山西长治 046000
5. 宝鸡市妇幼保健院检验科陕西宝鸡 721006
折叠

摘要

Abstract

Aim:To investigate the effects of silencing Transgelin-2 on the malignant biological behavior of esophageal squamous cell carcinoma(ESCC)cells infected by Porphyromonas gingivalis(PG)and its possible mechanism.Methods:NE6-C or KYSE140 cells were allocated into 4 groups and given the following treatments:transfected with siControl,PG in-fection(multiplicity of infection 10),siTAGLN-2 transfection,and siTAGLN-2 transfection +PG infection,respectively.Cell proliferation was detected by CCK-8,cell cycle was detected by BrdU mark,migration and invasion were detected by Tran-swell chamber assay,and the distribution of Transgelin-2,F-actin,E-cadherin and Vimentin proteins was detected by immu-nofluorescence assay,the protein expressions of Transgelin-2,E-cadherin and Vimentin were detected by Western blot.Re-sults:PG infection alone increased the proliferation rate of NE6-C and KYSE140 cells,increased the proportion of S-phase cells,the number of invasive cells and the number of migratory cells(P<0.05),and up-regulated Transgelin-2 expression(P<0.05),decreased F-actin;down-regulated E-cadherin expression and up-regulated Vimentin expression(P<0.05).siTAGLN-2 transfection significantly antagonized the above effects of PG infection(P<0.05).Conclusion:Transgelin-2 may inhibit the malignant progression of ESCC cells induced by PG infection through altering the cytoskeleton.

关键词

食管鳞状细胞癌/牙龈卟啉单胞菌/Transgelin-2/上皮间质转化/应力纤维

Key words

esophageal squamous cell carcinoma/Porphyromonas gingivalis/Transgelin-2/epithelial-mesenchymal transition/stress fiber

分类

医药卫生

引用本文复制引用

伍当柔,高社干,齐义军,朱巧晴,朱亚飞,许海军,陈攀,王明,赵云岗,孙丹妮,郭苒..Transgelin-2对牙龈卟啉单胞菌感染的食管鳞状细胞癌细胞恶性生物学行为的影响[J].郑州大学学报（医学版）,2023,58(6):741-745,5.

基金项目

河南省医学科技攻关省部共建重点项目(SBGJ202002100) （SBGJ202002100）

国家自然科学基金项目(81872037) （81872037）

郑州大学学报（医学版）

OA北大核心CSTPCD

ISSN：1671-6825

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