郑州大学学报（医学版）2023，Vol.58Issue(6)：757-763,7.DOI:10.13705/j.issn.1671-6825.2023.06.046
当归-川芎药对改善脑缺血再灌注急性损伤的代谢组学机制分析
Metabolomics mechanism study on the improvement of acute cerebral is-chemia/reperfusion injury by Danggui-Chuanxiong herb pair
摘要
Abstract
Aim:To explore the possible mechanism of Danggui-Chuanxiong herb pair against acute cerebral ischemia/reperfusion injury.Methods:A total of 60 female SD rats were randomly allocated into 6 groups,with 10 rats in each group.DG,CX and GX groups were treated with 5 times of clinical dose of Danggui,Chuanxiong and Danggui-Chuanxiong herb pair,respectively;while NM group was treated with Nimodipine 20 mg/(kg·d);Sham group and the model group were giv-en the same volume of normal saline by gavage for 7 days.Except the Sham group,the middle cerebral artery occlusion/reperfusion(MCAO/R)model was established by wire embolism in the other 5 groups on the 7th day.After 24 hours of reperfusion,serum and brain tissue samples were collected,ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-QTOF/MS)technology was used to perform non-targeted metabolomics analysis,and en-dogenous differential metabolites were screened by combining unsupervised principal component analysis(PCA),supervised partial least squares discriminant analysis(PLS-DA),and orthogonal partial least squares discriminant analysis(OPLS-DA),and metabolic pathway analysis annotations were performed.Results:There were significant differences in the meta-bolic profiles of serum and brain tissue between sham group and model group(P<0.05).A total of 19 potential biomarkers related to the MCAO/R model,including purines,phospholipids,and fatty acids,were identified through screening.There were 5 serum biomarkers and 14 brain biomarkers.Among them,there were 11 biomarkers closely related to the treatment of MCAO/R injury with Danggui-Chuanxiong herb pair,mainly related to ascorbate and aldarate metabolism,nicotinate and nicotinamide metabolism,glycerophospholipid metabolism,pentose and glucuronate interconversions,citrate cycle,ect.Con-clusion:Danggui-Chuanxiong herb pair can reverse metabolic disorders of the rats with acute cerebral ischemia/reperfusion injury,and the screened endogenous differential metabolites may be potential target for the treatment.关键词
当归-川芎药对/脑缺血再灌注损伤/代谢组学/UPLC-QTOF/MS/大鼠Key words
Danggui-Chuanxiong herb pair/cerebral ischemia/reperfusion injury/metabolomics/UPLC-QTOF/MS/rat分类
医药卫生引用本文复制引用
翟优,李伟霞,李琨,陈毓龙,泥文娟,纪秋如,王晓艳,张辉,孟伟亭,唐进法..当归-川芎药对改善脑缺血再灌注急性损伤的代谢组学机制分析[J].郑州大学学报（医学版）,2023,58(6):757-763,7.基金项目
国家自然科学基金项目(82004082,U1504827,82205081) （82004082,U1504827,82205081）
河南省科技研发计划联合基金优势学科培育类项目(222301420087) （222301420087）
河南省高校科技创新团队项目(23IRTSTHN026) （23IRTSTHN026）
河南省中医药拔尖人才培养项目(豫中医科教[2018]35号) （豫中医科教[2018]35号）
中国博士后科学基金特别资助项目(2023T160069) （2023T160069）
