郑州大学学报（医学版）2023，Vol.58Issue(6)：763-769,7.DOI:10.13705/j.issn.1671-6825.2022.05.052
lncRNA Meg3在全反式维甲酸和转化生长因子β3影响小鼠腭突间充质细胞增殖过程中的作用
Role of lncRNA Meg3 in proliferation of mouse embryonic palatal mesenchy-mal cells treated by all-trans retinoic acid or transforming growth factor β3
摘要
Abstract
Aim:To explore the role of long non-coding RNA(lncRNA)Meg3 in the proliferation of mouse embryonic palate mesenchymal cells treated by all-trans retinoic acid(atRA)or transforming growth factor β3(TGF-β3).Methods:Mouse embryonic palate mesenchymal cells were isolated and cultured on gestation day 13.EdU assay was used to detect the proliferation ability of mouse embryonic palate mesenchymal cells after treatment with 5 μmol/L atRA(atRA group)or 10 μg/L TGF-β3(TGF-β3 group)for48 hours.The cells not treated were used as control.The localization and relative expres-sion of Meg3 in the cells of the above 3 groups were detected by fluorescence in situ hybridization and real-time fluorescence quantification PCR.EdU assay was used to detect the effects of 5 μmol/L atRA and 10 μg/L TGF-β3 on the proliferation of mouse embryonic palate mesenchymal cells before and after Meg3 siRNA transfection.Results:Compared with control,at-RA significantly inhibited the proliferation of mouse embryonic palate mesenchymal cells,while TGF-β3 significantly promo-ted the proliferation;besides,atRA prompted the expression of Meg3 in mouse embryonic palate mesenchymal cells,while TGF-β3 inhibited the expression of Meg3(P<0.05).After Meg3 gene silencing,compared with control group,the prolifera-tion of mouse embryonic palate mesenchymal cells in the Meg3 siRNA group,atRA group and TGF-β3 group was signifi-cantly promoted(P<0.05).Conclusion:The inhibitive effects of atRA on palatal mesenchymal cell proliferation may be primarily mediated by Meg3,while the effects of TGF-β3 on the proliferation of palatal mesenchymal cells may not directly relate to Meg3 gene.关键词
长链非编码RNA Meg3/腭裂/腭突间充质细胞/全反式维甲酸/转化生长因子β3/小鼠Key words
long non-coding RNA Meg3/cleft palate/palatal mesenchymal cell/all-trans retinoic acid/transforming growth factor β3/mouse分类
医药卫生引用本文复制引用
刘小转,张军喜,余增丽,王国旭,何志东,宋帅星,李雪..lncRNA Meg3在全反式维甲酸和转化生长因子β3影响小鼠腭突间充质细胞增殖过程中的作用[J].郑州大学学报（医学版）,2023,58(6):763-769,7.基金项目
国家自然科学基金项目(81801547) （81801547）
河南省科技攻关省部共建重点项目(SBGJ202102014) （SBGJ202102014）
国家卫生健康委员会出生缺陷预防重点实验室&河南省人口缺陷干预技术研究重点实验室开放基金项目(ZD202203) （ZD202203）
